钒苷 B (Vanicoside B) 是一种苯丙基蔗糖衍生物，可从何首乌 (Persicaria dissitiflora) 中分离得到。钒苷 B 靶向周期蛋白依赖性激酶 8 (CDK8)并表现出抗肿瘤活性。其潜在机制是钒苷 B 阻断CDK8介导的信号通路，抑制上皮-间充质过渡蛋白的表达，从而导致细胞周期阻滞和凋亡 (apoptosis)。
| 生物活性 | Vanicoside B is a phenylpropanoyl sucrose derivative, can be isolated from the herbPersicaria dissitiflora. Vanicoside B targetscyclin-dependent kinase8(CDK8) and exhibits anti-tumor activity. The potential mechanism is Vanicoside B blocksCDK8-mediated signaling pathways and decreases the expression of epithelial-mesenchymal transition proteins, so that it leads to cell cycle arrest andapoptosis[1][2]. |
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体外研究
(In Vitro) | 钒苷 B (2.5-20 μM; 72 h) 对三阴性乳腺癌 (TNBC) MDA-MB-231 和 HCC38 癌细胞具有抗增殖活性[1]。
钒苷 B (2.5-20 μM; 72 h, 14 d 和 72 h) 抑制 TNBC 细胞的细胞活力、集落形成，干扰细胞周期[1]。
钒苷 B (2.5-10 μM; 48 h) 降低了 p-STAT1，p-STAT3 和 p-S6 的蛋白水平，并通过调节 Skp2-p27 系列蛋白表达而诱导 TNBC 细胞凋亡[1]。
Western Blot Analysis[1] | Cell Line: | MDA-MB-231 cells and HCC38 cells | | Concentration: | 0, 2.5, 5, 10 μM | | Incubation Time: | 48 hours | | Result: | Increased cleaved PARP, and p27 protein expressions, but decreased Skp2 protein level.
Suppressed CDK8 target genes and the expression of EMT-associated proteins.
Suppressed the expression of the cell proliferation marker Ki-67 in tumor tissues, also significantly suppressed the expressions of p-STAT1 (S727) and AXL. |
Cell Cycle Analysis[1] | Cell Line: | MDA-MB-231 cells and HCC38 cells | | Concentration: | 0, 2.5, 5, 10 μM | | Incubation Time: | 72 hours | | Result: | Inhibited cell cycle at sub-G1 phase. |
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体内研究
(In Vivo) | 钒苷 B (5 mg/kg 或 20 mg/kg; 腹腔注射; 每周 3 次, 共 4 周) 抑制 MDAMB-231 细胞移植的小鼠模型中的肿瘤生长[1]。
| Animal Model: | MDA-MB-231 cell-implanted xenograft mouse model[1] | | Dosage: | 5 mg/kg, 20 mg/kg | | Administration: | Intraperitoneal injection; 3 times per week over 4 weeks | | Result: | Significantly reduced tumor volumes at 5 mg/kg and 20 mg/kg by 53.85% and 65.72%, respectively. |
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| 来源 | - Plants
- other families
- Perilla ocimoidesL.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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