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Bafilomycin C1
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Bafilomycin C1图片
CAS NO:88979-61-7
包装与价格：
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议

产品名称
巴菲霉素C1
产品介绍
Bafilomycin C1 是从链霉菌中分离得到的大环内酯类抗生素 (antibiotic)。Bafilomycin C1 是一种有效的、特异性的、可逆的vacuolar-type H+-ATPases抑制剂。Bafilomycin C1 抑制革兰氏阳性菌 (bacteria) 和真菌 (fungi) 的生长。Bafilomycin C1 可诱导细胞凋亡 (apoptosis)，可用于肝细胞癌 (HCC) 的研究。
生物活性

Bafilomycin C1 is amacrolideantibioticisolated fromStreptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor ofvacuolar-type H+-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positivebacteriaandfungi[2]. Bafilomycin C1 induces cellapoptosisand can be used for the study of hepatocellular carcinoma (HCC)[2].

IC50& Target

Macrolide

 

体外研究
(In Vitro)

Bafilomycin C1 (0.33-10 μM; 6 days) inhibits the growth and proliferation of SMMC7721 and HepG2 cells in a timeand dose-dependent manner[2].
Bafilomycin C1 (0.33-3.3 μM; 24 hours) decreases cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 expression in both mRNA and protein expression in SMMC7721 cells[2].
Bafilomycin C1 (3.3-10 μM; 24 hours) causes morphological alterations and increases the population of apoptotic cells by Hoechst 33258 (HY-15558) staining compared to vehicle[2].

Cell Viability Assay[2]

Cell Line:SMMC7721 cell and HepG2 cell
Concentration:0.33 μM, 1.1 μM, and 3.3 μM for SMMC7721
1.1 μM, 3.3 μM, and 10.0 μM for HepG2
Incubation Time:6 days
Result:Retarded the cell growth.

Western Blot Analysis[2]

Cell Line:SMMC7721 cells
Concentration:3.3 μM
Incubation Time:24 hours
Result:Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21.

Apoptosis Analysis[2]

Cell Line:SMMC7721 and HepG2 cells
Concentration:3.3 μM; 10 μM
Incubation Time:24 hours
Result:Induced apoptosis in SMMC7721 and HepG2 cells.
体内研究
(In Vivo)

Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model[1].

Animal Model:BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×106cells/100 μL)[2]
Dosage:0.2 mg/kg
Administration:Subcutaneous injection; 20 days
Result:Suppressed tumor growth of SMMC7721 tumor xenografts.
分子量

720.89

性状

Solid

Formula

C39H60O12

CAS 号

88979-61-7

中文名称

巴菲霉素C1

结构分类
  • Antibiotics
  • Macrolide Antibiotics
来源

Streptomyces sp

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vivo:
  • 1.

    Bafilomycin C1 (0.2 mg/kg body weight, dissolved in 60% 1,2-propanediol)[2]