Okadaic acid

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Okadaic acid

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	78111-17-8
包装:	25 μg (124.2 μM * 250 μL in Ethanol)
市场价:	2300元

产品名称

冈田酸

产品介绍

Okadaic acid 是一种海洋毒素，是蛋白质磷酸酶 (PP) 的抑制剂，包括PP1(IC₅₀=15-50 nM)、PP2A(IC₅₀=0.1-0.3 nM)、PP3(IC₅₀=3.7-4 nM)、PP4(IC₅₀=0.1 nM) 和PP5(IC₅₀=3.5 nM)，其中对 PP2A 的亲和力最显著，但不抑制 PP2C。Okadaic acid 通过抑制 PP 增加了多种蛋白的磷酸化，并具有肿瘤启动子的作用。Okadaic acid 诱导 tau 蛋白磷酸化。

生物活性

Okadaic acid, a marine toxin, is an inhibitor of protein phosphatases (PP). Okadaic acid has a significantly higher affinity forPP2A(IC₅₀=0.1-0.3 nM), and inhibitsPP1(IC₅₀=15-50 nM),PP3(IC₅₀=3.7-4 nM),PP4(IC₅₀=0.1 nM),PP5(IC₅₀=3.5 nM), but does not inhibit PP2C. Okadaic acid increases of phosphorylation of a number of proteins by inhibiting PP, and acts a tumor promoter. Okadaic acid induces tau phosphorylation^[1]^[2].

IC₅₀& Target^[1]

PP1

15-50 nM (IC₅₀)

PP2A

0.1-0.3 nM (IC₅₀)

PP3

3.7-4 nM (IC₅₀)

PP4

0.1 nM (IC₅₀)

PP5

3.5 nM (IC₅₀)

PP2B

~4000 nM (IC₅₀)

PP7

＞1000 nM (IC₅₀)

体外研究
(In Vitro)

Okadaic acid (0-100 nM; 24 h or 48 h) inhibits the proliferation of AGS, MNK-45, Caco 2 cells^[3].
Okadaic acid (10 nM; 8 hours) increases Drp1 phosphorylation and mitochondrial fission in rat cortical neurons^[4].

Cell Proliferation Assay^[3]

Cell Line:	AGS, MNK-45 and Caco 2 cell lines
Concentration:	0-100 nM
Incubation Time:	24 h or 48 h
Result:	Inhibited the proliferation of AGS, MNK-45, Caco 2 cells.

体内研究
(In Vivo)

Okadaic acid (100 μM; injected unilaterally to the lateral amygdala) induces Tau phosphorylation and protein aggregation in anatomically distinct brain regions 24 h post-injection^[5].

分子量

805.00

性状

Liquid

Formula

C₄₄H₆₈O₁₃

CAS 号

78111-17-8

中文名称

冈田酸

结构分类

Ketones, Aldehydes, Acids

来源

marine mollusks?

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Solution, -20°C, 2 years