Pomalidomide-C3-adavosertib 是一种快速和选择性的Wee1降解剂 (IC50=3.58 nM)。Pomalidomide-C3-adavosertib 具有抗癌细胞增殖活性，并能诱导细胞凋亡。
| 生物活性 | Pomalidomide-C3-adavosertib is a rapid and selectiveWee1degrader (IC50=3.58 nM). Pomalidomide-C3-adavosertib shows anti-cancer cell proliferation activity, and inducesapoptosis[1]. |
体外研究
(In Vitro) | Pomalidomide-C3-adavosertib (1 nM-10 μM; 24, 48, and 72 h) induces CRBN-dependent anti-proliferative effects and synergized with Olaparib[1].
Pomalidomide-C3-adavosertib (100 nM; 24 h) induces DNA damage, apoptosis, and deregulation of the G2/M checkpoint[1].
Pomalidomide-C3-adavosertib (1 nM-10 μM; 6 h) degrades Wee1 in MOLT4 cells[1].
Cell Proliferation Assay[1] | Cell Line: | OVCAR8, COV362, and Kuramochi cells | | Concentration: | 1 nM-10 μM | | Incubation Time: | 24, 48, and 72 hours | | Result: | Degraded Wee1 in OVCAR8 cells, with maximal degradation observed at a 100 nM treatment. |
Cell Cycle Analysis[1] | Cell Line: | MOLT4 cells | | Concentration: | 100 nM | | Incubation Time: | 24 hours | | Result: | Led to reduced pCDKl Y15, increased apoptosis, and increased unrepaired DNA. |
Western Blot Analysis[1] | Cell Line: | MOLT4 cells | | Concentration: | 1 nM-10 μM | | Incubation Time: | 6 hours | | Result: | Degraded Wee1 after a 6-hour treatment in MOLT4 cells, and induced the downstream changes expected from Wee1 loss-including a decrease in phosphorylated cyclin-dependent kinase 1 (pCDK1) and an increase in pH 3. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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