SPHINX

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SPHINX

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

848057-98-7

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
25mg	电议
50mg	电议
100mg	电议
200mg	电议

产品介绍

SPHINX 是SRPK1的选择性抑制剂，IC₅₀值为 0.58 μM。SPHINX 有效地减少体内脉络膜新生血管 (CNV)。SPHINX 可用于老年性黄斑变性的研究。

生物活性

SPHINX is a selectiveSRPK1inhibitor with anIC₅₀value of 0.58 μM. SPHINX effectively reduces Choroidal Neovascularization (CNV) in vivo. SPHINX can be used for the research of (age-related macular degenaration) AMD^[1].

IC₅₀& Target

IC50: 0.58 μM (SRPK1)^[1]

体外研究
(In Vitro)

SPHINX (10 μM; 2 h) affects EGF-induced phosphorylation of SRSF1 and SRSF2^[1].
SPHINX (5 μM; 24 h) reduces the expression of VEGF165 relative to GAPDH control either in primary RPE and ARPE-19 cell lines^[1].

Western Blot Analysis^[1]

Cell Line:	ARPE-19 cell line
Concentration:	10 μM
Incubation Time:	2 hours
Result:	Blocked EGF-induced phosphorylation of SRSF1 and SRSF2.

体内研究
(In Vivo)

SPHINX (10 ng; i.o. on laser photocoagulation day 0 and day 7) affects neovascular growth in vivo^[1].
SPHINX (25 ng; i.o. on laser photocoagulation day 0 and day 7) affects the CNV area in CNV rats^[1].

Animal Model:	C57/B6 mice with laser-induced CNV^[1]
Dosage:	10 ng
Administration:	Intraocular injection; 10 ng on laser photocoagulation day 0 and day 7
Result:	Significantly reduced neovascular growth compared with saline-injected controls.

Animal Model:	Norway Brown rats with laser-induced choroidal neovascularization^[1]
Dosage:	25 ng (10 ng/uL)
Administration:	Intraocular injection; 25 ng (10 ng/uL) on laser photocoagulation day 0 and day 7
Result:	Significantly reduced the CNV area compared with saline injected controls.

Clinical Trial

分子量

354.32

性状

Solid

Formula

C₁₇H₁₇F₃N₂O₃

CAS 号

848057-98-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 50 mg/mL(141.12 mM;ultrasonic and warming and heat to 60℃)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.8223 mL	14.1115 mL	28.2231 mL
5 mM	0.5645 mL	2.8223 mL	5.6446 mL
10 mM	0.2822 mL	1.4112 mL	2.8223 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。