Ciliobrevin D is a cell-permeable, reversible and specific inhibitor of AAA+ ATPase motorcytoplasmic dynein. Ciliobrevin D inhibitsHedgehog(Hh)signaling and primary cilia formation. Ciliobrevin D inhibitsdynein-dependent microtubule gliding and ATPase activity in vitro^[1][2].

Cytoplasmic dynein^[1][2]

Cells treated with Ciliobrevin D exhibits abnormal (unfocused, multipolar, or collapsed) spindles with disrupted γ-tubulin localization in NIH-3T3 cells. Similar Ciliobrevin-induced spindle defects are observed in HeLa cells, although to a lesser extent. Ciliobrevin D addition also reversibly disrupts the pre-formed spindles of metaphase-arrested cells and reduces overall microtubule levels^[1].
. Ciliobrevin D reversibly inhibits melanosome aggregation, but the non-cilia-disrupting derivative had no discernible effect at comparable doses. Ciliobrevin D similarly abrogates the movement of peroxisomes in Drosophila S2 cells^[1].

Knockdown of Dync1h1 or inactivation of dynein 1 by Ciliobrevin D in the testis in vivo perturbs spermatogenesis. Knockdown of Dync1h1 or the use of Ciliobrevin D to inactivate dynein 1 in the testis in vivo perturbs MT organization through changes in the spatial expression of EB1, perturbs F-actin organization, and perturbs distribution of adhesion protein complexes at the BTB, leading to a loss of BTB integrity. F-actin disorganization in the seminiferous epithelium following Dync1h1 knockdown or dynein 1 inactivation by Ciliobrevin D is mediated by changes in the spatiotemporal expression of actin regulatory proteins Arp3 and Eps8^[3].

392.62

Solid

C₁₇H₈Cl₃N₃O₂

1370554-01-0

Room temperature in continental US; may vary elsewhere.

DMSO : 5 mg/mL(12.73 mM;ultrasonic and warming and heat to 80℃)

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