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[Tyr8] Bradykinin
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
[Tyr8] Bradykinin图片
CAS NO:32222-00-7
包装与价格：
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
[Tyr8] Bradykinin 是一种B2激肽受体 (B2kinin receptor) 激动剂。[Tyr8] Bradykinin 也刺激ERK1/2磷酸化。[Tyr8] Bradykinin 可作为内标。
生物活性

[Tyr8] Bradykinin is aB2kinin receptoragonist. [Tyr8] Bradykinin also stimulatesERK1/2 phosphorylation. [Tyr8] Bradykinin can be used as an internal standard[1].

IC50& Target

Bradykinin B2 Receptor (B2R)

 

ERK1

 

ERK2

 

体外研究
(In Vitro)

[Tyr8] Bradykinin (10 nM, 2 min) produces a rapid increase in phosphorylated ERK1/2[1].

Western Blot Analysis[1]

Cell Line:Serum-deprived trabecular meshwork cells
Concentration:10 nM
Incubation Time:2 min
Result:Produced a rapid increase in phosphorylated ERK1/2 that was equivalent in magnitude to that observed forBradykinin(HY-P0206).
分子量

1076.21

Formula

C50H73N15O12

CAS 号

32222-00-7

Sequence Shortening

RPPGFSPYR

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign value
Acidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1
Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1
Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetails
Negative (<0)1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.