Hoechst 33258 analog is an analogue of the Hoechst dye. Hoechst 33258 analog is a cellular dye that can be used to quantify DNA. Hoechst 33258 analog emits blue fluorescence when bound to dsDNA^[1].

Hoechst 33258 analog is used to detect the presence of nucleic acids in cells. Hoechst 33258 analog specifically to the target DNA sequence and emits blue fluorescence when bound to dsDNA. Hoechst 33258 analog is conjugated to a reporter probe and a signal generator^[1].

Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells^[1].

510.59

Solid

C₂₉H₃₀N₆O₃

258843-62-8

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DMSO : ≥ 51 mg/mL(99.88 mM)

*"≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

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  Description: Hoechst 33258 analog can be used to stain DNA for detecting apoptosis.
  Method: For cell staining.
  1. 48 h after transfection, cell slides are prepared and fixed in 4% paraformaldehyde.
  2. Stain cells with Hoechst 33258 analog (30 min; dark) solution.
  3. Use a fluorescence microscope (Nikon Instruments Inc., Tokyo, Japan) for image.