DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.

DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry^[1].

350.25

Solid

C₁₆H₁₇Cl₂N₅

28718-90-3

4′,6-二脒基-2-苯基吲哚

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

DMSO : 75 mg/mL(214.13 mM;Need ultrasonic)

H₂O : 5.56 mg/mL(15.87 mM;ultrasonic and warming and heat to 60℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (5.94 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20%SBE-β-CDin saline)

  Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (5.94 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (5.94 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• 
  Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.
  Method: For cell staining.
  1. Cells are cultured in Dulbecco’s modified Eagle medium (DMEM), and supplemented with 10% fetal bovine serum.
  2. Cells are expanded in an incubator for 12 h at 37℃ with 5% CO₂and then seeded onto confocal dishes.
  3. Cells are grown until confluence and washed with Hank’s balanced salt solution (HBSS).
  4. Cells are stained with DAPI dihydrochloride and imaged by a confocal laser scanning microscopy.
• 
  Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.
  Method: For cell staining (TUNEL assay).
  1. Mice are sacrificed, and their small intestine tissues are harvested and fixed with 4% formaldehyde.
  2. The tissue sections are incubated with a TUNEL reaction mixture (1 h; 37℃; dark).
  3. The sections are stained with DAPI dihydrochloride (0.1 μg/mL).
  4. Cells are observed under an inverted fluorescence microscope (Olympus, Japan).
• 
  Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.
  Method: For cell staining.
  1. Incubate cultured cells in DAPI dihydrochloride (1 μg/mL; 5 min).
  2. Observe staining cells under a fluorescence microscope.
• 
  Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.
  Method: For cell staining.
  1. Wash cultured cells for three times with PBS, cells are mounted with medium containing DAPI dihydrochloride.
  2. Samples are imaged with an Axioskop 2 mot plus fluorescence microscope equipped with Plan Apochromat 203/0.8 NA and 403/0.95 NA objectives and an AxioCam MRc camera with AxioVision software 4.7.1 (Carl Zeiss AG, Jena, Germany).