Cell lines

CCRF-CEM cells

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Reaction Conditions

IC 50: 3.3 μM, 1 hour

Applications

Amrubicin induced DNA-protein complex formation in cultured CCRF-CEM cells in a dose-dependent manner. The IC50 value of amrubicin required to inhibit the growth of CCRF-CEM cells was 3.3 μM. Accordingly, under conditions where cell growth was inhibited by amrubicin, considerable amounts of DNA-protein complexes were formed.

Animal models

BALB/c nu/nu mice bearing Lu-24 or Lu-134 cells

Dosage form

Intravenous injection, 25 mg/kg

Applications

Amrubicin showed significant antitumor activities against both SCLC tumors tested, Lu-24 and Lu-134, with T/C-values at day 14 of 17% and 9%, respectively.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

A synthetic anthracycline antibiotic. It inhibits DNA topoisomerase II. Antineoplastic.