Temozolomide(TMZ)

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Temozolomide(TMZ)

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	85622-93-1
包装:	10mg, 50mg, 100mg, 500mg

包装与价格：

产品名称

NSC362856
CCRG81045

产品介绍

Temozolomide (TMZ)是一种DNA损伤诱导剂。Temozolomide是一种甲基化剂，可穿过血脑屏障，适用于恶性胶质瘤和转移性黑色素瘤。

分子量	194.15
分子式	C₆H₆N₆O₂
CAS号	85622-93-1
纯度	99.86%
溶解性（25°C）	DMSO 18 mg/mL
储存和运输条件	-20°C, protect from light, sealed 常温运输及临时存放

细胞实验
细胞系	A2058, A375, M238 and M249 cell lines
方法	Colony formation assay Depending on the cell line (and plating efficiency), 200–1000 cells were seeded into each well of a 6-well plate and treated as described in detail previously. In the case of primary melanoma cells, 1000 cells were seeded and let grow for 24 days in the presence or absence of drug treatment; because only small colonies formed, the stained colonies were counted under the microscope.Survival of melanoma cells after drug treatment. Five different melanoma cell lines (as indicated), as well as a culture of primary melanoma tissue cells (labeled ‘primary’) were exposed to increasing concentrations of TMZ (diamonds) or NEO212 (circles) for 48 hours, and long-term survival was determined via colony formation assay (CFA).
浓度	0, 25, 50, 75, 100μM
处理时间	48 h

动物实验
动物模型	A375 cells subcutaneous tumor growth model
配制	45% glycerol, 45% ethanol, 10% DMSO
剂量	50 mg/kg
给药处理	subcutaneous injection

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.1507 mL	25.7533 mL	51.5066 mL
5 mM	1.0301 mL	5.1507 mL	10.3013 mL
10 mM	0.5151 mL	2.5753 mL	5.1507 mL