Arachidonoyl glycine-d₈(NAGly-d₈) contains eight deuterium atoms at the 5, 6, 8, 9, 11, 12, 14, and 15 positions. It is intended for use as an internal standard for the quantification of NAGly by GC-or LC-mass spectrometry. NAGly has been isolated from cell cultures treated with arachidonoyl ethanolamide (AEA),¹from extracts of mammalian brain,^2,3and has also been synthesized as an analog of AEA for structure/activity testing.⁴NAGly may be produced endogenouslyviaoxidation of AEA, or by transacylation of arachidonoyl coenzyme A. NAGly is reported to have analgesic activities in whole animal experiments.^1,2,3Since it seems to be a very poor ligand for the CB₁receptor,⁴these effects are probably mediatedviaother signalling pathways.

1.Burstein, S.H., Rossetti, R.G., Yagen, B., et al.Oxidative metabolism of anandamideProstaglandins & Other Lipid Mediators6129-41(2000) 2.Huang, S.M., Bisogno, T., Petros, T.J., et al.Identification and characterization of an endogenous anandamide-like compound: N-arachidonylglycine (NAGly)ICRS 2001 Symposium on the Cannabinoids78(2001) 3.Huang, S.M., Bisogno, T., Petros, T.J., et al.Identification of a new class of molecules, the arachidonyl amino acids, and characterization of one member that inhibits painThe Journal of Biological Chemisty276(46)42639-42644(2001) 4.Sheskin, T., Hanus, L., Slager, J., et al.Structural requirements for binding of anandamide-type compounds to the brain cannabinoid receptorJournal of Medicinal Chemistry40659-667(1997)