Ganetespib

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Ganetespib

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	888216-25-9
包装:	5mg, 10mg, 50mg, 100mg

包装与价格：

产品名称

STA-9090

产品介绍

Ganetespib (STA-9090)是一种HSP90抑制剂，作用于OSA 8种细胞，IC50为4 nM，诱导OSA细胞凋亡，而对正常的成骨细胞无作用；是STA-1474的活性代谢物。

细胞实验
细胞系	NCI-H1975 and HCC827 cell lines
方法	Cell viability assays. Cells were grown in 96-well plates based on optimal growth rates determined empirically for each line. Twenty-four hours after plating, cells were treated with the indicated compounds or controls for 72 hours. AlamarBlue (Invitrogen) was added (10% v/v) to the cells, and the plates were incubated for 3 hours and, then, subjected to fluorescence detection. For the comparative viability/ apoptosis assay, NCI-H1975 cells were treated with escalating concentrations of ganetespib for the indicated time periods and subjected to viability analysis via CellTiter Fluor (Promega) and apoptosis via Caspase Glo 3/7 (Promega).
浓度	0~10 μM
处理时间	6, 24, 48, and 72 hours

动物实验
动物模型	NCI-H1975 tumor xenograft-implanted SCID mice
配制	formulated in 10/18 DRD (10% dimethyl sulfoxide, 18% Cremophor RH 40, 3.6% dextrose, 68.4% water)
剂量	125 mg/kg for 6-72 hours
给药处理	oral gavage

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.7442 mL	13.7212 mL	27.4424 mL
5 mM	0.5488 mL	2.7442 mL	5.4885 mL
10 mM	0.2744 mL	1.3721 mL	2.7442 mL