TAK-901

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TAK-901

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	934541-31-8
包装:	2mg, 5mg, 10mg, 50mg

包装与价格：

产品名称

TAK901

产品介绍

TAK-901是一种新型Aurora A/B抑制剂，IC50为21 nM/15 nM，不是有效的细胞JAK2， c-Src和Abl抑制剂。

细胞实验
细胞系	A2780, A375, A549, COLO-205, HCT116, PC-3 cells line
方法	Cell proliferation and viability assays. Cells were plated in 96-well microtiter plates and incubated with serial dilutions of TAK-901 for 72 hours. Cell proliferation was determined by ELISA analysis of bromodeoxyuridine (BrdUrd) incorporation into DNA (Roche Diagnostics). IMR-90 immortalized lung fibroblasts were seeded in 96-well microtiter plates and cultured for 3 to 4 days until confluent. Cells were then incubated with serial dilutions of TAK-901 for 72 hours. The MTS assay was conducted as per the manufacturer's instructions (Promega). The concentration of TAK-901 required to inhibit half of the maximal effective concentration (EC50) was determined from the dose–response curves.
浓度	0~8 μM
处理时间	72 h

动物实验
动物模型	Nude mice or rats Xenograft models
配制	12% Captisol, 25 mmol/L citrate, pH 3.0
剂量	5 μL/mg twice daily
给药处理	intravenously

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.9816 mL	9.9081 mL	19.8161 mL
5 mM	0.3963 mL	1.9816 mL	3.9632 mL
10 mM	0.1982 mL	0.9908 mL	1.9816 mL