A fluorogenic substrate for CYPs
货号:ajcx25462
CAS:575-04-2
分子式:C11H7F3O3
分子量：244.2
溶解度:DMF: 30 mg/ml,DMF:PBS (pH 7.2) (1:2): 0.3 mg/ml,DMSO: 25 mg/ml,Ethanol: 10 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

7-Methoxy-4-(trifluoromethyl)coumarin is a fluorogenic substrate for cytochrome P450s (CYPs).¹Upon enzymatic cleavage by CYPs, 7-hydroxy-4-(trifluoromethyl)coumarin (HFC) is released and its fluorescence can be used to quantify CYP activity. HFC displays excitation/emission maxima of 410/510 nm, respectively.

1.Donato, M.T., JimÉnez, N., Castell, J.V., et al.Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymesDrug Metab. Dispos.32(7)699-706(2004)