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Substance P(1-7)(TFA)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Substance P(1-7)(TFA)图片
规格:98%
分子量:1014.06
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品介绍
Substance P (1-7)(TFA) 是神经肽 P 物质 (SP) 的片段。Substance P (1-7)(TFA) 可对孤束核产生抑制和缓和作用。
货号:ajcx14138
CAS:N/A
分子式:C43H66F3N13O12
分子量:1014.06
溶解度:H2O: 50 mg/mL (49.31 mM)
纯度:98%
存储:Store at -20°C
库存:现货

Background:

Substance P (1-7)(TFA) is a fragment of the neuropeptide, substance P (SP). Substance P (1-7)(TFA) gives depressor and bradycardic effects when applied to the nucleus tractus solitarius.


Substance P (1-7) is found to act as a very potent antagonist against the SP-induced responses and is formed locally in the nigra after SP injection. It is proposed that Substance P (1-7) is an endogenous modulator of SP actions[1]. Injection of low doses of Substance P (1-7) (1.0-4.0 pM simultaneously with SP or SP(5-11) (0.1 nM), reduce aversive behaviours induced by SP or SP(5- 11) significantly. These results indicate that SP(1-7) formed endogenously could modulate the actions of SP or SP(5-11) in the spinal cord[2].

[1]. Herrera-Marschitz M, et al. The substance P(1-7) fragment is a potent modulator of substance P actions in the brain. Brain Res. 1990 Jun 25;521(1-2):316-20. [2]. Sakurada T, et al. Substance P(1-7) antagonizes substance P-induced aversive behaviour in mice. Neurosci Lett. 1988 Dec 19;95(1-3):281-5.

Protocol:

Animal experiment:

Rats[1]Sprague-Dawley male rats weighing 250-300 g are anaesthetized with halothane and placed in a stereotaxic frame. An injection cannula, conically shaped with a penetration tip diameter of approximately 0.15 mm is loared into the SNR. Saline (0.2 μL), SP (0.007-0.7 nmol) or Substance P (1-7) (0.01-1 nmol) is injected into the left substantia nigra, pars reticulata (SNR) and the rat is placed in a rotometer. The substances are injected in a total volume of 0.2 μL over a period of 1 min. A group of animals is sacrificed by decapitation 1 hour after the injection, their brains are immediately removed and tissue samples are taken from left and right striatum, globus pallidum (GP) and substantia nigra (SN). Samples are assayed for SP and SP(1-7)[1].Mice[2]The accumulated response time (s) of reciprocal movements of hindlimb scratching, biting, fore- and hindpaw licking are measured in Male mice (STD strain, 23-28 g) during the whole period of aversive response and 20 min at maximum. Substance P (1-7) is tested for its ability to inhibit the aversive response produced by intrathecal injection of SP or SP(5-11) (0.1 nM/mouse). Substance P (1-7) (1, 2, 4 pmol) is then administered together with SP or SP(5-11)[2].

参考文献:

[1]. Herrera-Marschitz M, et al. The substance P(1-7) fragment is a potent modulator of substance P actions in the brain. Brain Res. 1990 Jun 25;521(1-2):316-20.
[2]. Sakurada T, et al. Substance P(1-7) antagonizes substance P-induced aversive behaviour in mice. Neurosci Lett. 1988 Dec 19;95(1-3):281-5.