| CAS NO: | 300586-90-7 |
| 规格: | ≥98% |
| 包装 | 价格(元) |
| 5mg | 电议 |
| 10mg | 电议 |
| 25mg | 电议 |
| 50mg | 电议 |
| 100mg | 电议 |
| 250mg | 电议 |
| 500mg | 电议 |
| Molecular Weight (MW) | 237.26 |
|---|---|
| Formula | C14H11N3O |
| CAS No. | 300586-90-7 |
| Storage | -20℃ for 3 years in powder form |
| -80℃ for 2 years in solvent | |
| Solubility (In vitro) | DMSO: 47 mg/mL (198.1 mM) |
| Water: <1 mg/mL | |
| Ethanol: 21 mg/mL (88.5 mM) | |
| Other info | Chemical Name:
N-(1H-pyrrolo[2,3-c]pyridin-5-yl)benzamide InChi Key: HWJRIFZDXJKJJN-UHFFFAOYSA-N InChi Code: InChI=1S/C14H11N3O/c18-14(10-4-2-1-3-5-10)17-13-8-11-6-7-15-12(11)9-16-13/h1-9,15H,(H,16,17,18) SMILES Code: O=C(NC1=CC2=C(NC=C2)C=N1)C3=CC=CC=C3 |
| Synonyms | BAS-00287861; BAS00287861; BAS 00287861; OAC 1; OAC1; OAC-1 |
| In Vitro | In vitro activity: OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, OAC1 enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using OAC1 along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. OAC1 may be used to enhance the reprogramming of somatic cells to a pluripotent state. Cell Assay: The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment. |
|---|---|
| In Vivo | In mouse embryonic fibroblasts (MEFs) with the 4F reprogramming quartet Oct4, Klf4, Sox2, and c-Myc, OAC1 enhanced reprogramming efficiency and increased the number of colonies with ESC-like morphology by twofold or more. Also, OAC1 increased induced pluripotent stem cells (iPSC)-like colonies. OAC1 had no effects on the Wnt signaling and p53-p21 expression. In MEFs, OAC1 activated the expression of endogenous Oct4 and Nanog. Also, OAC1 increased the expression of Sox2 and Tet1. |
| Animal model | |
| Formulation & Dosage | |
| References | Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20853-8. |
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