| CAS NO: | 127191-97-3 |
| 规格: | ≥98% |
| 包装 | 价格(元) |
| 5mg | 电议 |
| 10mg | 电议 |
| 25mg | 电议 |
| 50mg | 电议 |
| 100mg | 电议 |
| 250mg | 电议 |
| 500mg | 电议 |
| Molecular Weight (MW) | 721.84 |
|---|---|
| Formula | C38H35N5O6S2 |
| CAS No. | 127191-97-3 |
| Storage | -20℃ for 3 years in powder form |
| -80℃ for 2 years in solvent | |
| Solubility (In vitro) | DMSO: 100 mg/mL (138.5 mM) |
| Water:<1 mg/mL | |
| Ethanol: <1 mg/mL | |
| Other info | Chemical Name: (S)-4-(2-(N-methylisoquinoline-5-sulfonamido)-3-oxo-3-(4-phenylpiperazin-1-yl)propyl)phenyl isoquinoline-5-sulfonate SMILES Code: CN([C@@H](CC1=CC=C(OS(=O)(C2=C3C(C=NC=C3)=CC=C2)=O)C=C1)C(N4CCN(C5=CC=CC=C5)CC4)=O)S(C6=CC=CC7=C6C=CN=C7)(=O)=O |
| Synonyms | KN62; KN-62; KN 62. |
| In Vitro | In vitro activity: KN-62 suppresses the A23187-induced autophosphorylation of Ca2+/CaM kinase in PC12 D cells. KN-62 (10 μM) inhibits carbachol and potassium-stimulated insulin secretion from rat pancreatic islets. KN-62 also inhibits growth of K562 cells and blocks cell cycle progression. Kinase Assay: Total kinase activity of CaMKII, determined in a standard 2 min assay (100 μL), contained 35 mM HEPES, 10 mM MgCl2, 1 mM CaCl2, 10 μg of chicken gizzard myosin 20-kD light chain, 0.1 μM calmodulin, and 10 μM [γ-33]ATP at 30 °C. The kinase reaction is halted by adding 1 mL of 10% trichloroacetic acid. Cell Assay: For cell growth analysis, K562 cells are plated in a 3-cm dish with 5 mL of culture medium containing various concentration of KN-62. After two days in these condition cell numbers are counted. |
|---|---|
| In Vivo | KN-62 inhibits seizure-induced expression of brain-derived neurotrophic factor mRNA in adult rat brain. |
| Animal model | Sprague Dawley Rats |
| Formulation & Dosage | Dissolved in 0.5 mM KN-62/50% DMSO; 2 pmol; intracerebroventricular (ICV) injection |
| References | J Biol Chem. 1990 Mar 15;265(8):4315-20; Eur J Neurosci. 1998 Jan;10(1):377-87. |
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