DIBA-Cy5 is a fluorescent DIBA antagonist made up be DIBA-alkyne binding Cyanine5 fluorophores (Cy5) and polyethylene glycol (PEG) biomolecules. DIBA-Cy5 can serve as a fluorescent ligand, suitable for probe attachment throughclick chemistry. DIBA-Cy5 exerts a high binding affinity totype-2mAChR(M2R) with the K_dvalue of 1.80 nM, can directly stain M2R receptors in the sinoatrial node of a mouse heart^[1].

DIBA-Cy5 (0.01 nM-1 mM; 5 h) shows selective binding property for type-2 mAChR (M₂R) over M₁R, with K_dof 1.08 nM and 104.5 nM, respectively^[1].
DIBA-Cy5 (10 nM; 3 h) exerts the competitive binding mode with orthosteric antagonistAtropine(HY-B1205)/allosteric modulatorLY2119620(HY-15885), indicating a dualsteric binding mode of the DIBA-type antagonist to M2R^[1].
DIBA-Cy5 (50 nM; 16 h) results direct staining to M2R receptors in the sinoatrial node of a mouse heart. DIBA-Cy5 can be used binding assays that screen compounds for M2R as the receptor target^[1].

1360.71

C₆₉H₈₉N₁₁O₁₂S₃

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