N6-Benzyladenosine 是一种腺苷受体激动剂，具有细胞分裂素 (cytokinin) 活性。N6-Benzyladenosine 能将细胞周期阻滞在 G0/G1，并诱导凋亡 (apoptosis)。N6-Benzyladenosine 显示出对弓形虫腺苷激酶和胶质瘤 (glioma) 的抑制作用。
| 生物活性 | N6-Benzyladenosine is anadenosine receptoragonist, has a cytoactive activity. N6-Benzyladenosine arrests cell cycle at G0/G1 phase and induces cellapoptosis. N6-Benzyladenosine also exerts inhibitory effect onT. gondiiadenosine kinaseand glioma[1]-[5]. |
体外研究
(In Vitro) | N6-benzyladenosine suppresses the clonogenic activity and the growth of different neoplastic cells[2].
N6-benzyladenosine results cell morphology alteration and actin cytoskeleton disorganization in T24 cell[2].
N6-benzyladenosine (10 μM; 24 h) is a potent inductor of apoptosis, and belongs to apoptotic systems with distinct caspase-3 and caspase-9 activation[3].
N6-benzyladenosine (0-100 μM; 24 h) induces chromatin condensation, formation of apoptotic bodies, and cleavage of DNA to nucleosomal fragments in a dose-dependent manner[3].
N6-benzyladenosine acts as a selective anti-toxoplasma agent with binding affinity toT. gondiiadenosine kinase (apparent Km=179.8 μM), over human adenosine kinase[4].
N6-benzyladenosine (0-50 μM) shows weak inhibition against adenosine kinase deficient (TgAKS3) strains of Toxoplasma gondii[4].
N6-benzyladenosine (compound 2) (0.3-20 μM) exerts anti-glioma activity by interfering with the mevalonate pathway and inhibiting FPPS (Farnesyl pyrophosphate synthase)[5].
Apoptosis Analysis[3] | Cell Line: | HL-60 | | Concentration: | 10 μM | | Incubation Time: | 24 hours | | Result: | Induced cell apoptosis by increasing caspase-3 (DEVDase) as well as caspase-9 (LEHDase) activity, indicating an apoptotic systems with distinct caspase-3/9 activation. |
Apoptosis Analysis[5] | Cell Line: | U87MG human glioma cell line. | | Concentration: | 0.3, 0.6, 1.2, 2.5, 5, 10, 20 μM | | Incubation Time: | 48 hours | | Result: | Inhibited glioma growth by interfering with the mevalonate pathway and inhibiting FPPS. |
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| 来源 | - Plants
- Umbelliferae
- Pimpinella anisumL.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : 125 mg/mL(349.79 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.7983 mL | 13.9915 mL | 27.9830 mL | | 5 mM | 0.5597 mL | 2.7983 mL | 5.5966 mL | | 10 mM | 0.2798 mL | 1.3991 mL | 2.7983 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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