DAF-FM DA is a reagent to detect and quantify low concentrations of nitric oxide (NO); DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.

496.42

Solid

C₂₅H₁₈F₂N₂O₇

254109-22-3

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

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  Description: DAF-FM DA is a fluorescence NO probe, it can be used for the detection of intracellular NO release.
  Method: For intracellular NO release detection.
  1. Culture cells until cells are adherent.
  2. Stain cells with DAF-FM DA solution (5 μM; 1 h; 37℃).
  3. Use an inverted fluorescence microscopy for image.