Dnp-PLGLWA-DArg-NH2 TFA is a fluorogenic substrate forMMP-1andMMP-9. Dnp-PLGLWA-DArg-NH2 TFA can be used to quantify the activity of MMPs (Ex=280 nm, Em=360 nm)^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[2].
1. Enzyme activity assays are performed in 50 mmol/l Tris-HCl buffer, pH 7.5, 0.15 mol/l NaCl, 10 mmol/l CaCl₂, 0.02% TNC buffer containing 0.05% Brij 35 and 50 μM ZnSO₄.
2. Dnp-PLGLWA-DArg-NH2 TFA. Each fraction is incubated with 1 μM substrate at 37℃ for 20 h.
3. Stop the reaction by the addition of 3% acetic acid.
4. Measure the fluorescence using wavelengths of 280 nm (excitation) and 360 nm (emission) with a fluorescence reader.

1091.10

C₄₇H₆₅F₃N₁₄O₁₃

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.