Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal,bacterialand fungal)^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability^[1][2]:
1. Defrost the Resazurin solution in a 37℃ water bath.
2. Place the cells on a 96-well plate, and washed by PBS (avoid light).
3. Remove the PBS wash, then add500 μL(1μg/mL) of Resazurin solution.
4. Place the plate, in the incubator for30 min(incubation time depends on cell type and cell number).
5. Measure Resorufin fluorescence (Ex=530-560 nm, Em=590 nm) using a microplate reader.

229.19

C₁₂H₇NO₄

550-82-3

刃天青

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.