生物制药 HPLC 分析->N-糖样品前处理和分析->外切糖苷酶
AdvanceBio Sialidase C，1 U. 酶从寡糖、糖蛋白、复杂碳水化合物中水解释放 α(2-3,6)-连接的唾液酸。包括 5x 反应缓冲液 B [250 mmol/L 磷酸钠 pH 6.0]。
| 体积 | 100 µL |
| 分子量 | ~41,000 daltons |
| 单位 | 1 U |
| ** pH 值 | 6 |
| 浓度 | 10 U/mL |
| 酶单位定义 | One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5 |
| 酶来源 | Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24. Sialidases are also known as neuraminidases. |
| 酶比活性 | ≥40 units/mg |
| 酶特异性 | This enzyme releases a2-3, and a2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc a2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. Sialidase A (GK80040) is recommended for this application. |
| 酶配方 | 20 mM Tris HCl pH 7.5, containing 25 mM NaCl |
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