| CAS: | 9047-57-8 | |
| 英文名称: |
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| 用途: | 来自肝素黄杆菌的软骨素酶AC是一种通过消除机制将己糖胺和葡萄糖醛酸残基之间具有(1-4)键的硫酸化和非硫酸化多糖链裂解的酶。 所得到的寡糖产物主要是具有不饱和糖醛酸的二糖。 软骨素酶AC可特异性地降解硫酸软骨素A和C,但不可降解硫酸软骨素B(硫酸皮肤素)。 |
| 货号 | 品牌 | 产品名称 | 规格 | 包装、参考价格 | 详情 | ||||||||||||||||||||||||||||||||
| C920567 | MACKLIN | 软骨素酶AC | ≥200 units/mg protein, For Chondroitin Sulfate Analysis | 5776元/10U; | 展开 | ||||||||||||||||||||||||||||||||
储存:−20°C 状态:白色粉末 | |||||||||||||||||||||||||||||||||||||
| C2780 | Sigma-Aldrich | Chondroitinase AC from Flavobacterium heparinum | lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate) | 5702.53元/5 UNITS; | 展开 | ||||||||||||||||||||||||||||||||
产品说明 应用 Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements. 包装 5 units in serum bottle 生化/生理作用 Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate). 其他说明 Contains potassium phosphate buffer salts and BSA as stabilizer. 适用性 Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides 单位定义 One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 ℃. Reaction volume: 3.1 ml (light path 1 cm). 制备说明 Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution. 基本信息
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| E2039 | Sigma-Aldrich | Chondroitinase AC from Flavobacterium heparinum | recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis | 7931.28元/10 UNITS; | 展开 | ||||||||||||||||||||||||||||||||
产品说明 应用 已经过高度纯化以去除干扰性β-葡糖醛酸糖苷酶和蛋白酶活性,可用于硫酸软骨素水解和 HPLC 分析。 β-葡萄糖醛酸酶活性:≤2,000 飞摩尔/分钟/mg 蛋白质,蛋白酶活性:≤2,000 飞摩尔/分钟/mg 蛋白质 单位定义 在37℃,pH 6.7条件下,1单位是指每分钟从硫酸软骨素A中释放1.0μmole不饱和双糖所需的酶量(通过A232的变化进行测定)。εμΜ值对于反应产物Δ-Di-4S(硫酸软骨素A和B)来说为5.1,对于Δ-Di-6S(硫酸软骨素C)来说为5.5。 其他说明 查看有关 用于复合碳水化合物分析的酶 的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer 基本信息
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