产品说明

一般描述

蜡状芽孢杆菌的鞘磷脂酶(Bc-Smase)是一种含Mg²⁺的金属酶。 蜡状芽孢杆菌 的鞘磷脂酶诱导绵羊红细胞的溶血。
鞘磷脂酶调节成纤维细胞和巨噬细胞大囊泡不依赖三磷酸腺苷(ATP)的内吞作用。鞘磷脂酶引起人红细胞溶血。 它也是导致侵入性疾病的有效毒力因子。

应用

来自 蜡样芽孢杆菌 的鞘磷脂酶已被用于：

• 测定脑片中的膜电容
• 在酶活性测定中检测并证实其杀蚕活性
• 用于阐明其对复合脂类混合物中鞘磷脂水解的影响

包装

10, 50 units in serum bottle

生化/生理作用

启动形成基于鞘磷脂的第二信使。激活 MAPK(丝裂原活化蛋白激酶)和 SAPKs(压力激活蛋白激酶)；从鞘磷脂生成神经酰胺。

单位定义

在 pH 7.8、25℃ 条件下，1 个单位将每分钟水解1.0μmolTNPAL-鞘磷脂。

外形

冻干粉，含磷酸钾缓冲盐和稳定剂。

基本信息

产品性质

安全信息

象形图
警示用语：	Warning
危险声明	H317
预防措施声明	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类	Skin Sens. 1
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)

产品说明

一般描述

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

应用

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.
Sphingomyelinase from Bacillus cereus has been used:

• in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
• in lipoprotein modification for the generation of cholesterol crystals in vitro
• to aggregate LDL (low-density lipoprotein)

生化/生理作用

启动形成基于鞘磷脂的第二信使。激活 MAPK(丝裂原活化蛋白激酶)和 SAPKs(压力激活蛋白激酶)；从鞘磷脂生成神经酰胺。
Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.

单位定义

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 ℃.

外形

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

基本信息

产品性质

安全信息

产品说明

应用

Sphingomyelinase from Staphylococcus aureus has been used to:

• induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
• determine the concentration of sphingomyelin from serum samples
• enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis

包装

25, 50 units in glass bottle

生化/生理作用

Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

单位定义

One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 ℃.

外形

Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5

基本信息

产品性质

安全信息