产品说明

一般描述

α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).

应用

α-Ketoglutarate Dehydrogenase from the porcine heart has been used:

• to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
• to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
• as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons

生化/生理作用

α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.

质量

可能含有痕量的聚乙二醇。

单位定义

在 30 ℃、pH 7.4、饱和水平的辅酶 A 存在的条件下，一单位每分钟可将 1.0 μ 摩尔的 β-NAD 转化成 β-NADH。

外形

以 50％ 甘油溶液的形式提供，含有 9mg 每毫升的牛血清白蛋白、30％ 蔗糖、1.5 mM EDTA、1.5 mM EGTA、1.5 mM 2-巯基乙醇、0.3％ TRITON^™ X-100、0.003％ 叠氮化钠和 15 mM 磷酸钾，pH 6.8。

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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