| CAS: | 9028-76-6 | ||
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| 货号 | 品牌 | 产品名称 | 规格 | 包装、参考价格 | 详情 | ||||||||||||||||||||||||||||||||||||
| C832435 | MACKLIN | 胆固醇氧化酶 | BR，10-20u/mg | 318元/200U; 948元/1KU; 3368元/5KU; | 展开 | ||||||||||||||||||||||||||||||||||||
储存：-20°C 状态：黄色粉末 | |||||||||||||||||||||||||||||||||||||||||
| 167589 | J&K | Cholesterol oxidase, from E.coli, ≥50 units/mg protein | 来源于大肠杆菌, ≥50 units/mg protein | 1654元/100UN; | 展开 | ||||||||||||||||||||||||||||||||||||
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| C8868 | Sigma-Aldrich | Cholesterol Oxidase from microorganisms | lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli | 2540.76元/100 UNITS; 8637.16元/500 UNITS; | 展开 | ||||||||||||||||||||||||||||||||||||
产品说明 应用 胆固醇氧化酶用于测定血清胆固醇。该酶还可应用于食品样品中类固醇的微量分析以及区分 3-酮类固醇和 3β-羟基类固醇。表达胆固醇氧化酶的转基因植物正在与棉铃象甲作斗争中进行研究。CHOD 也被用作分子探针来阐明细胞膜结构。 包装 100, 500 units in glass bottle 生化/生理作用 胆固醇氧化酶 (CHOD) 是一种含有 FAD 的单体黄素蛋白，其催化胆固醇分解代谢的第一步。这种双功能酶在需要 FAD 的步骤中将胆固醇氧化为胆甾-5-烯-3-酮，然后随着H2O2的释放而异构化为胆甾-4-烯-3-酮。 物理属性 含 FAD 辅因子的 Ⅱ 型胆固醇氧化酶与该酶共价连接。 单位定义 在 pH 值为 7.5、25℃ 条件下，每分钟 1 个单位的胆固醇将转化为 4-胆甾烯-3-酮 (1.0 μmol/min)。注：4-胆甾烯-3-酮可能发生异构化。 外形 以含有蔗糖的冻干粉末形式提供 基本信息
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| C5421 | Sigma-Aldrich | Cholesterol Oxidase from microorganisms | aqueous solution, ≥30 units/mg protein (biuret) | 2754.95元/100 UNITS; 10908.6元/500 UNITS; | 展开 | ||||||||||||||||||||||||||||||||||||
产品说明 应用 Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites. 生化/生理作用 Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 ℃. The pH stability is 5.7-7.8. Hg2+, Ag+, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage. 单位定义 One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 ℃. Note: 4-cholesten-3-one may undergo isomerization. 制备说明 CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use. 基本信息
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| C8649 | Sigma-Aldrich | Cholesterol Oxidase from Streptomyces sp. | lyophilized powder, ≥20 units/mg protein | 3041.42元/100 UNITS; 6045.35元/250 units; | 展开 | ||||||||||||||||||||||||||||||||||||
产品说明 一般描述 由链霉菌产生的胆固醇氧化酶分子量为62kDa。它的最适温度和pH分别为37 ℃和7。胆固醇氧化酶包含FAD结合和类固醇结合结构域。 应用 来自链霉菌的胆固醇氧化酶已用于评估模拟胆汁的胶束结构与酯酶活性之间的关系。来自链霉菌的胆固醇氧化酶还被用于研究鞘磷脂降解对细胞胆固醇氧化性以及细胞表面与细胞内部之间稳态分布的影响。 包装 100, 250 units in glass bottle 生化/生理作用 来自微生物的胆固醇氧化酶利用胆固醇获取碳和能量。它可用于涉及胆固醇的诊断测定，特别是脂质异常。胆固醇氧化酶具有杀虫性能，有助于防止棉铃象鼻虫幼虫的侵袭。链霉菌胆固醇氧化酶保存期长，并且具有良好的性能。 物理属性 等电点：5.1±0.1和5.4±0.1 单位定义 一个酶活性单位是指在pH 7.5、25℃下，每分钟将1.0 μM胆固醇转化为4-胆甾烯-3-酮所需的酶量。注意：4-胆甾烯-3-酮可能会发生异构化。 外形 冻干粉末，含有牛血清白蛋白和糖作为稳定剂 制备说明 CHOD溶于冰冷的50 mM磷酸钾缓冲液，pH7.0。临用前配制。 基本信息
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| C1235 | Sigma-Aldrich | Cholesterol Oxidase microbial | recombinant, lyophilized powder, ≥10 units/mg protein | 2863.39元/100 UNITS; | 展开 | ||||||||||||||||||||||||||||||||||||
产品说明 应用 Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures. 包装 100 units in glass bottle 生化/生理作用 Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H2O2. Optimum pH of the enzyme is 7.0. Hg2+, Ag+, ionic detergents inhibit the enzyme activity. 单位定义 One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 ℃ and pH 7.0 in a peroxidase linked system. 制备说明 Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use. 基本信息
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| 228250 | Sigma-Aldrich | Cholesterol Oxidase, Streptomyces sp. | Cholesterol Oxidase, Streptomyces sp. CAS 9028-76-6, is a native cholesterol oxidase that catalyzes the oxidation of cholesterol to cholesterone and hydrogen peroxide. | 7007.84元/500 U; | 展开 | ||||||||||||||||||||||||||||||||||||
产品说明 一般描述 Native cholesterol oxidase from Streptomyces species. Catalyzes oxidation of cholesterol to cholesterone and hydrogen peroxide (isoelectric points: 6.1 and 7.3). Suitable for determination of total cholesterol when coupled with cholinesterase and peroxidase. Has an optimal pH of 6.0-8.0. 包装 500 u in Plastic ampoule 警告 Toxicity: Standard Handling (A) 单位定义 One unit is defined as the amount of enzyme that will oxidize 1.0 µmol of cholesterol per min at 25°C, pH 7.0. 其他说明 Gadda, G., et al. 1997. Eur. J. Biochem. 250, 369. 法律信息 CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany 基本信息
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