| CAS: | 9026-12-4 | ||||||||||||||||||||
| 分子量: | 384.41 | ||||||||||||||||||||
| 英文名称: |
| ||||||||||||||||||||
| 用途: | Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA. |
| 货号 | 品牌 | 产品名称 | 规格 | 包装、参考价格 | |||||||||||||||||||||||||||||||
| R863691 | MACKLIN | 核糖核酸酶T1 来源于米曲霉(色谱纯化) | ≥300,000 units/mg protein | 721元/100KU; | 咨询 | ||||||||||||||||||||||||||||||
储存：2-8°C 状态：无色到浅黄色到浅米黄色液体 | |||||||||||||||||||||||||||||||||||
| R796641 | MACKLIN | 核糖核酸酶T1 | 来源于米曲霉, 色谱纯化,冻干 | 926元/100KU; | 咨询 | ||||||||||||||||||||||||||||||
储存：2-8°C | |||||||||||||||||||||||||||||||||||
| R1003 | Sigma-Aldrich | Ribonuclease T1 from Aspergillus oryzae | ammonium sulfate suspension, 300,000-600,000 units/mg protein | 1143.01元/100000 UNITS; 5137.71元/500000 UNITS; | 咨询 | ||||||||||||||||||||||||||||||
产品说明 应用 来自 米曲霉的核糖核酸酶T1(RNase T1)可用于在测序之前对变性的RNA进行消化并用于蛋白折叠的研究 。 生化/生理作用 来自米曲霉的核糖核酸酶T1(RNase T1)是一种内切核糖核酸酶，其可在G残基后进行水解。切割发生在胍核糖核苷酸的3'-磷酸基团和相邻核苷酸的5'-羟基之间。初始产物为2'：3'环磷酸核苷，其可水解成相应的3'-核苷磷酸。它与胰腺RNase的不同之处在于它可特异性攻击鸟嘌呤位点以产生具有3′-GMP末端基团的3′-GMP和寡核苷酸。 单位定义 在pH7，5,37℃条件下，一个单位可在15分钟内产生相当于 ΔA260 为 1.0 的酸可溶性寡核苷酸，反应体积为1.0 mL。底物：酵母RNA。 外形 2.8 M (NH4)2SO4 溶液混悬液 分析说明 E 基本信息
产品性质
安全信息
| |||||||||||||||||||||||||||||||||||
m.cnreagent.com