| CAS: | 9026-00-0 | ||||||
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| 用途: | The enzyme is found primarily in pancreas and pancreatic secretions, but in other tissues as well. Bile salts, such as cholate and its conjugates, act as stabilizers of the native polymeric form of the enzyme and can protect it from proteolytic degradation in the intestine. |
| 货号 | 品牌 | 产品名称 | 规格 | 包装、参考价格 | |||||||||||||||||||||||||||||||||||
| C864257 | MACKLIN | 胆固醇酯酶 来源于猪胰腺 | ≥300 units/g dry weight | 1008元/100U; 3058元/500U; 5219元/1KU; | 咨询 | ||||||||||||||||||||||||||||||||||
储存：-20°C 状态：冻干粉末 | |||||||||||||||||||||||||||||||||||||||
| C964353 | MACKLIN | 胆固醇酯酶 来源于荧光假单胞菌 | lyophilized powder, ≥10,000 units/g protein | 950元/10U; 2120元/50U; 5200元/250U; | 咨询 | ||||||||||||||||||||||||||||||||||
储存：-20°C 状态：冻干粉末 | |||||||||||||||||||||||||||||||||||||||
| C798923 | MACKLIN | 胆固醇酯酶 来源于假单胞菌 | >15U/mg | 514元/1KU; 1554元/5KU; 4339元/25KU; | 咨询 | ||||||||||||||||||||||||||||||||||
储存：-20°C 状态：白色到浅黄色到浅棕色粉末或晶体 | |||||||||||||||||||||||||||||||||||||||
| C3766 | Sigma-Aldrich | Cholesterol Esterase from bovine pancreas | lyophilized powder, ≥200 units/g protein | 7797.14元/100 UNITS; 29887.7元/500 UNITS; | 咨询 | ||||||||||||||||||||||||||||||||||
产品说明 一般描述 Cholesterol Esterase from bovine pancreas is a member of α-β hydrolase superfamily. The core of the protein comprises 11 β-strands, surrounded by 15 α-strands. Catalytic triad is in the centre of the molecule. The C-terminus possesses hydrophobic residues. 应用 Cholesterol Esterase from bovine pancreas has been used:
Cholesterol esterase bound to membrane-associated heparin on brush border membranes aids in the transport of cholesterol and free fatty acid across the membrane. This enzyme is widely used in the determination of serum cholesterol in clinical laboratories. The enzyme from Sigma has been used to evaluate the inhibitory and antioxidant functions of the methanol extract of the Camellia sinensis leaves under in vitro conditions. The enzyme has also been used to digest human serum samples to confirm the presence and position of acyl esters of 7α-hydroxycholesterol. Enzyme responsible for the hydrolysis of many of the fatty acid esters of cholesterol. Optimum pH range: 6-8 Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg2+, sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine 包装 100, 500 units in poly bottle 生化/生理作用 Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. This monomeric glycoprotein may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is found to be 84 kDa. 其他说明 部分纯化 单位定义 One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per minute at pH 7.0 at 37 ℃ in the presence of taurocholate. 外形 This product is partially purified from bovine pancreas and is supplied as a white to light brown lyophilized powder containing ≥20% protein (biuret) and potassium phosphate. 制备说明 Cholesterol esterase is soluble in 0.4 M potassium phosphate, pH 7.0 at 1 mg/mL concentration. 分析说明 Protein determined by biuret. 基本信息
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| C1403 | Sigma-Aldrich | Cholesterol Esterase from Pseudomonas sp. | lyophilized powder, ≥200,000 units/g protein | 3414.93元/25 UNITS; 10295.8元/100 UNITS; | 咨询 | ||||||||||||||||||||||||||||||||||
产品说明 应用 当在临床分析中与胆固醇氧化酶结合使用时，该酶可用于酶法测定总胆固醇。 生化/生理作用 胆固醇酯酶(CE)是一种可逆酶，可以水解或合成胆固醇和其他固醇的脂肪酸酯。水不溶性长链脂肪酸酯的水解需要经过胆盐活化。短链脂肪酸和溶血磷脂的水溶性酯的水解则不需要胆盐的活化。它还可水解三、二和单酰基甘油、磷脂、溶血磷脂和神经酰胺。该酶在脂质和脂蛋白代谢以及动脉粥样硬化中可能具有多种功能。 物理属性 稳定性：在–20℃稳定至少一年 单位定义 在牛磺胆酸盐存在下，一个单位在 pH7.0，37℃ 下每分钟将 1.0μmole胆固醇油酸酯水解成胆固醇和油酸。 基本信息
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| C9281 | Sigma-Aldrich | Cholesterol Esterase from Pseudomonas fluorescens | lyophilized powder, ≥10,000 units/g protein | 3328.91元/100 UNITS; 13296.9元/500 UNITS; | 咨询 | ||||||||||||||||||||||||||||||||||
产品说明 应用 来自 荧光假单胞菌 的胆固醇酯酶已用于含胆固醇氧化酶的酶促胆固醇试剂组分的优化研究。荧光假单胞菌产生的胆固醇酯酶也用于研究脂解制剂酯酶活性的非变性蛋白质电转移。 包装 100, 500 units in poly bottle 生化/生理作用 胆固醇酯酶 (CE) 是一种能水解或合成胆固醇及其他甾醇脂肪酸酯的可逆性酶。水不溶性长链脂肪酸酯的水解需要胆盐活化。短链脂肪酸和溶血磷脂的水溶性酯水解不需要胆盐激活。也水解三、二和单酰基甘油、磷脂、溶血磷脂和神经酰胺类。这种酶可能在脂质和脂蛋白代谢以及动脉粥样硬化中具有多种功能。其分子量约为 129 kDa，最适 pH 范围为 7.0-9.0。该酶可被胆酸、甘胆酸、BSA、Mg 2 + 和 0.3% (v/v) ™ X-100 活化。Ag + 、Hg 2 + 和离子型洗涤剂抑制酶的活性。 其他说明 含有磷酸钾和 TRITON®X-100。 单位定义 在牛磺胆酸盐存在下，一个单位在 pH7.0，37℃ 下每分钟将 1.0μ 摩尔胆固醇油酸酯水解成胆固醇和油酸。 分析说明 双缩脲法测定蛋白质。 法律信息 Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow 基本信息
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| 26745 | Sigma-Aldrich | Cholesterol Esterase from porcine pancreas | lyophilized, powder, white, ~35 U/mg | 1897.69元/10 MG; 11183.7元/100 MG; | 咨询 | ||||||||||||||||||||||||||||||||||
产品说明 一般描述 Cholesterol Esterase is a serine hydrolase released from the exocrine pancreas. It belongs to the α/β hydrolase family of enzymes. 应用 Cholesterol Esterase from porcine pancreas has been used:
生化/生理作用 For the hydrolysis of cholesterol esters in the determination of total cholesterol in serum or plasma; Application in (selective) hydrolysis/condensation of carboxylic ester bonds 单位定义 1 U相当于在pH 7.0和37℃下每分钟释放1μmol胆固醇的酶的量(乙酸胆固醇为底物)。 其他说明 可能适用相应的销售限制 基本信息
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