产品说明

应用

牛脾脱氧核糖核酸酶Ⅱ已用于：

• 作为从结肠样品分离免疫细胞的消化混合液组分
• 消化视网膜外植体以制备单细胞悬液
• 分离人结肠组织的基质细胞
• 作为从大鼠心脏制备胚胎心肌细胞的解离培养基组分

包装

30000, 150000, 300000 units in poly bottle

生化/生理作用

脱氧核糖核酸酶II，也称为酸性DNA酶，可水解天然和变性DNA中的脱氧核糖核苷酸连接键，产生带有3′-磷酸的产物。体外最佳pH范围是4.5-5.0。它还可在pH 5.6-5.9下作用于对硝基苯基磷酸二酯。分子量约为38,000 Da。

单位定义

在 pH 4.6 25℃条件下，一个Kunitz单位可每分钟在每毫升内产生0.001的ΔA₂₆₀；[Mg²⁺] = 0.83 mM

基本信息

产品性质

安全信息

产品说明

应用

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.
DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).

包装

20000, 80000 units in poly bottle

生化/生理作用

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.

单位定义

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25℃; [Mg²⁺] = 0.83 mM

外形

含氯化钠

基本信息

产品性质

安全信息