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CAS: 9014-19-1
英文名称: 
pyruvate carboxylase from bovine liver
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货号品牌产品名称规格包装、参考价格
P996317MACKLIN丙酮酸羧化酶buffered aqueous glycerol solution, 5-25 units/mg protein(BCA)17777元/10U;   咨询
储存:-20°C, 密封, 干燥
状态:液体
P7173Sigma-AldrichPyruvate Carboxylase from bovine liverbuffered aqueous glycerol solution, 5-25 units/mg protein (BCA)10335.7元/10 UNITS;   咨询

产品说明

应用

Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.

生化/生理作用

Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.

单位定义

One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 ℃.

外形

Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.

制备说明

亲和纯化

基本信息

酶学委员会(EC)编号6.4.1.1 ( BRENDA | IUBMB )
MDL编号MFCD00132143
NACRESNA.26

产品性质

形式buffered aqueous glycerol solution
质量水平200
specific activity5-25 units/mg protein (BCA)
浓度≥0.5 mg/mL
异质活性lactic dehydrogenase ≤0.5%
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable
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