| 货号 | 品牌 | 产品名称 | 规格 | 包装、参考价格 | 详情 |
| B802762 | MACKLIN | 5-溴-2'-脱氧尿苷 | 99% | 33元/250mg; 99元/1g; 376元/5g; 1504元/25g; | 展开 |
储存：-20°C 状态：白色到黄色粉末或晶体 折射率：22 ° (C=1, H2O) |
| B792848 | MACKLIN | 5-溴-2'-脱氧尿苷 | 10mM in DMSO | 368元/1ml; | 展开 |
储存：-20°C, 避光 状态：白色至黄色粉末或晶体 |
| B1575 | TCI | 5-Bromo-2'-deoxyuridine | >98.0%(T)(HPLC) | 290元/1G; 990元/5G; | 展开 |
基本信息 | 纯度/分析方法 | >98.0%(T)(HPLC) | | 分子式/分子量 | C9H11BrN2O5 = 307.10 | | 外观与形状(20℃) | 固体 | | 储存在惰性气体下 | 存放于惰性气体之中 | | 应避免的情况 | 空气 | | Reaxys-RN | 4236087 | | PubChem物质ID | 87564478 | | MDL编号 | MFCD00006529 |
技术规格 | Appearance | White to Almost white powder to crystal | | Purity(HPLC) | min. 98.0 area% | | Purity(Neutralization titration) | min. 98.0 % | | Specific rotation [a]20/D | +20.0 to +24.0 deg(C=1, H2O) | | Solubility in hot Water | within almost transparency |
物性(参考值) 相关法规 |
| 579031 | J&K | 5-Bromo-2'-deoxyuridine | 99%, 一种核苷类似物,可与胸苷竞争掺入DNA,5-BrdU通常用于检测增殖细胞 | 304元/500MG; 551元/1G; 1982元/5G; | 展开 |
基本信息 | 分子式 | C9H11BrN2O5 | | 分子量 | 307.10 | | 熔点 | 191-194 | | MDL编码 | MFCD00006529 | | Beilstein | 30395 | | Merck | 14,1452 | | EINECS 编号 | 200-415-9 | | 存储条件 | Freezer -20℃ |
产品描述 Bromodeoxyuridine (BrdU)是一种核苷类似物，与胸腺嘧啶竞争性整合到DNA，用于增生细胞的检测。 靶点（IC50 & Targe） Others, 体外研究 在RG2大鼠胶质瘤细胞中，Bromodeoxyuridine对癌细胞系和癌症干细胞数扩增诱导进行性，剂量响应抑制。在H9细胞和BJ纤维母细胞中，Bromodeoxyuridine改变细胞周期分布。[1]BrdU稳定整合到DNA，因此可以用于细胞增殖和其他细胞过程的评估。[2] 体内研究 在大鼠胶质瘤RG2肿瘤模型中，Bromodeoxyuridine (300 mg/kg, i.p. 或0.8 mg/ml, p.o.)显著减慢肿瘤形成。[1] 细胞实验 Cell lines: RG2大鼠胶质瘤细胞 Concentrations: ～ 50 μM Incubation Time: 24小时 Method: 细胞首先以2000 细胞/cm2接种到培养基，然后用Z2 Coulter计数器定量。RG2大鼠胶质瘤细胞用0，1，10，或50μM BrdU处理24小时，累积增长曲线在18天后得到。将对照组和处理的细胞定量，在处理后第5，12，和18天以相同密度再接种。 (Only for Reference) 参考文献 [1] Levkoff LH, et al. Neoplasia. 2008, 10(8), 804-816. [2] Rothaeusler K, et al. Curr Protoc Cytom. 2007, Chapter 7, Unit 安全信息 | 图形或危害标志 |  | | 提示语 | Danger | | 危险说明 | H340
H361 | | 防范说明 | P201
P281
P308+P313 | | WGK | 2 | | RTECS | YU7350000 | | TSCA | 是 |
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| H27260 | Alfa Aesar | 5-Bromo-2'-deoxyuridine | 99% | 970元/250mg; 2672元/1g; 8517元/5g; | 展开 |
应用
5-Bromo-2'-deoxyuridine (BrdU) is a thymidine analog used to label DNA. It is incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies to target the BrdU incorporation. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation. 备注
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Store away from strong oxidizing agents. 基本信息 MDL
MFCD00006529 EINECS
200-415-9 分子式
C9H11BrN2O5 分子量
307.10 熔点
ca 190° dec. 灵敏度
Keep Cold. 形态
Crystalline powder 溶解性
Soluble in water. Beilstein
30395 Merck
14,1452 GHS危害和防范说明 GHS符号
Hazard Statements
H341
Suspected of causing genetic defects. Precautionary Statements
P201-P280-P202-P308+P313-P405-P501a
Obtain special instructions before use.Wear protective gloves/protective clothing/eye protection/face protection.Do not handle until all safety precautions have been read and understood.IF exposed or concerned: Get medical advice/attention.Store locked up.Dispose of contents/container in accordance with local/regional/national/international regulations. 安全信息 危险类别
68
Possible risk of irreversible effects. 安全等级
36/37
Wear suitable protective clothing and gloves. RTECS
YU7350000 TSCA
是 |
| BP25085 | Alfa Aesar | 5-BROMO-2'-DEOXYURIDINE 5GM | | 3285元/5g; | 展开 |
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| BP2508250 | Alfa Aesar | 5-BROMO-2'-DEOXYURIDINE 250MG | | 796元/250mg; | 展开 |
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| BP2508500 | Alfa Aesar | 5-BROMO-2'-DEOXYURIDINE 500MG | | 1399元/500mg; | 展开 |
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| BP25081 | Alfa Aesar | 5-BROMO-2'-DEOXYURIDINE 1GM | | 1685元/1g; | 展开 |
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| 203806 | Millipore | 5-Bromo-2'-deoxyuridine-CAS 59-14-3-Calbiochem | Thymidine analog. Useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. | 1931.33元/1 G; 7086.17元/5 G; | 展开 |
产品说明 一般描述 Thymidine analog. Useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Can be used both in vitro and in vivo to label DNA. The resulting DNA can be used in conjunction with BrdU (Ab-2) Monoclonal Antibody (Cat. No. NA20) for the immunohistochemical analysis of cell proliferation.
Thymidine analog. Used for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Can be used both in vitro and in vivo to label DNA. The resulting DNA can be used in conjuction with BrdU (Ab-2) Monoclonal Antibody (Cat. No. NA20) or BrdU Immunohistochemical System (Cat. No. HCS24) for the immunohistochemical analysis of cell proliferation. 包装 1, 5 g in Plastic ampoule 警告 Toxicity: Harmful & Carcinogenic / Teratogenic (E) 重悬 Following reconstitution, sterilize by filtration through a 0.22 µm filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. 其他说明 Garabedian, E.M., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 15382.
Cattoretti, G., et al. 1993. J. Pathol. 171, 83.
Fukuda, K., et al. 1990. Anal. Quant. Cytol. Histol. 12, 135. 法律信息 CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany 基本信息 产品性质 | 质量水平 | 100 | | 测定 | ≥98% (HPLC) | | 形式 | solid | | manufacturer/tradename | Calbiochem® | | 储存条件 | OK to freeze | | 颜色 | white | | 溶解性 | water: 10 mg/mL
0.1 M NaOH: 50 mg/mL
DMSO: 50 mg/mL | | 运输 | ambient | | 储存温度 | 15-25℃ | | InChI | 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16) | | InChI key | WOVKYSAHUYNSMH-UHFFFAOYSA-N |
安全信息 | 象形图 | | | 警示用语： | Danger | | 危险声明 | H340 - H361fd | | 预防措施声明 | P201 - P202 - P280 - P308 + P313 - P405 - P501 | | 危险分类 | Muta. 1B - Repr. 2 | | 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects | | WGK | WGK 2 |
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| 10280879001 | Roche | 5-Bromo-2′-deoxyuridine | >97%, crystalline solid, pkg of 1 g | 1144.87元/1 G; | 展开 |
产品说明 一般描述 通常用作 DNA 的 S 期合成的标记物。 应用 可被免疫化学检测到的核苷酸，可代替胸苷掺入 DNA 中。根据姐妹染色单体交换 (SCE) 方法，用于体内 DNA 合成研究 和致突变物质检测。
5-溴-2′-脱氧尿苷已用于在细胞增殖研究中标记细胞周期S期细胞和原代肝细胞。 生化/生理作用 用作遗传研究中诱变剂的胸苷类似物。在 S 期可选择性结合到细胞 DNA 中。 质量 纯度 ：97%(来自 N)，色谱均一 制备说明 工作浓度： 据文献报道用于细胞培养，终浓度为 10μmol/l。
据文献报道，5～50 mg/kg 体重小鼠已用于细胞增殖的检测。
工作溶液的制备
对应于 Roche 细胞增殖 ELISA、BrdU 试剂盒和 Roche 5-溴-2′ 中包含的 BrdU 标记试剂-脱氧尿苷标记和检测试剂盒，将物质溶于 PBS 中，制备 BrdU 工作液至 10 mM 储备液(BrdU 的分子量 = 307.1 D)。对于 体内 使用， BrdU 溶解在 PBS 中；对于 体外 BrdU 的使用，溶于双蒸水中，浓度与 10 mM 相同。
储存条件(工作液)：-15 至-25℃ 其他说明 仅用于生命科学研究。不可用于诊断。 基本信息 | 经验(实验)分子式 | C9H11BrN2O5 | | 分子量 | 307.10 | | Beilstein | 30395 | | MDL编号 | MFCD00006529 | | PubChem化学物质编号 | 329749004 |
产品性质 | 质量水平 | 100 | | 测定 | >97% | | 形式 | crystalline solid | | 分子量 | 307.1 | | 包装 | pkg of 1 g | | manufacturer/tradename | Roche | | mp | 191-194 ℃ (dec.) (lit.) | | 运输 | wet ice | | 储存温度 | 2-8℃ | | SMILES string | OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O | | InChI | 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1 | | InChI key | WOVKYSAHUYNSMH-RRKCRQDMSA-N |
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| B5002 | Sigma-Aldrich | 5-Bromo-2′-deoxyuridine | ≥99% (HPLC) | 735.46元/100 MG; 928.65元/250 MG; 1406.96元/500 MG; 1726.72元/1 G; 6870元/5 G; 89666.9元/100 G; | 展开 |
产品说明 应用 5-溴-2′-脱氧尿苷用于：
- 用作研究电痉挛性癫痫对海马神经发生的影响的补充剂
- 用作研究斑马鱼成鱼基因丧失功能的补充剂
- 心脏T淋巴细胞增殖试验{133]
5-溴-2′-脱氧尿苷(5-BrdU)是一种可插入到DNA中的胸苷类似物。 5-BrdU日常被广泛用于对DNA合成进行测量以及对分裂的细胞进行标记。 因此，5-BrdU用于研究细胞信号传导和诱导细胞增殖的其他过程。 包装 1, 5, 100 g in glass bottle
100, 250, 500 mg in glass bottle 生化/生理作用 5-溴-2′-脱氧尿苷(BrdU)用于分析细胞增殖，因为它在细胞周期的S期可轻松掺入DNA。BrdU刺激白血病细胞系中的细胞分化和成熟，同时抑制Friend红白血病细胞分化。随后通过诸如流式细胞分析或荧光显微分析方法研究掺入BrdU时，通常使用带有荧光标记的BrdU特异性抗体来检测掺入的BrdU。
用作遗传研究中诱变剂的胸苷类似物。在 S 期可选择性结合到细胞 DNA 中。 基本信息 | 经验(实验)分子式 | C9H11BrN2O5 | | 分子量 | 307.10 | | Beilstein | 30395 | | EC 号 | 200-415-9 | | MDL编号 | MFCD00006529 | | PubChem化学物质编号 | 24278270 | | NACRES | NA.51 |
产品性质 | 质量水平 | 200 | | 测定 | ≥99% (HPLC) | | 形式 | powder | | mp | 191-194 ℃ (dec.) (lit.) | | 溶解性 | DMSO: 50 mg/mL, clear, colorless to very faintly yellow | | 储存温度 | −20℃ | | SMILES string | OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O | | InChI | 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1 | | InChI key | WOVKYSAHUYNSMH-RRKCRQDMSA-N |
安全信息 | 象形图 | | | 警示用语： | Danger | | 危险声明 | H340 - H361fd | | 预防措施声明 | P201 - P202 - P280 - P308 + P313 - P405 - P501 | | 危险分类 | Muta. 1B - Repr. 2 | | 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects | | WGK | WGK 2 | | 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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| B9285 | Sigma-Aldrich | 5-Bromo-2′-deoxyuridine | BioUltra, ≥99% | 722.13元/50 MG; 1263.07元/250 MG; 3791.86元/1 G; | 展开 |
产品说明 一般描述 5′-溴-2-脱氧尿苷(BrdU)是一种可被皮肤吸收或吸入的致癌物质。BrdU溶液对光敏感。 应用 5-溴-2′-脱氧尿苷已用于：
- 在细胞增殖测定中标记妊娠小鼠的增殖细胞
- 研究缺血后不同时间的细胞增殖时程确认MKI67 +细胞在细胞周期中重新进入生殖细胞
- 成纤维细胞5′-溴-2′脱氧尿苷(BrdUrd)染色
包装 50, 250 mg in poly bottle
1 g in poly bottle 生化/生理作用 5-溴-2′-脱氧尿苷(5-BrdU)是一种胸苷类似物，可掺入DNA中。 5-BrdU通常广泛用于测定DNA合成和标记分裂细胞。 因此，5-BrdU常用于研究细胞信号传导和诱导细胞增殖的其他过程。
5′-溴-2-脱氧尿苷(BrdU)染色主要用于体内观察肿瘤细胞和其他组织的增殖。
用作遗传研究中诱变剂的胸苷类似物。在 S 期可选择性结合到细胞 DNA 中。 基本信息 | 经验(实验)分子式 | C9H11BrN2O5 | | 分子量 | 307.10 | | Beilstein | 30395 | | EC 号 | 200-415-9 | | MDL编号 | MFCD00006529 | | PubChem化学物质编号 | 24892132 | | NACRES | NA.51 |
产品性质 | 产品线 | BioUltra | | 质量水平 | 200 | | 测定 | ≥99% | | 形式 | powder | | 杂质 | ≤0.005% Phosphorus (P)
≤0.1% Insoluble matter | | 灼烧残渣 | ≤0.1% | | mp | 191-194 ℃ (dec.) (lit.) | | 溶解性 | NH4OH: 0.1 M at 20 ℃, clear, colorless | | 痕量阳离子 | Al: ≤0.0005%
Ca: ≤0.01%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005% | | 储存温度 | −20℃ | | SMILES string | OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O | | InChI | 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1 | | InChI key | WOVKYSAHUYNSMH-RRKCRQDMSA-N |
安全信息 | 象形图 | | | 警示用语： | Danger | | 危险声明 | H340 - H361fd | | 预防措施声明 | P201 - P202 - P280 - P308 + P313 - P405 - P501 | | 危险分类 | Muta. 1B - Repr. 2 | | 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects | | WGK | WGK 2 | | 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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| 092742 | Fluorochem | 5-Bromo-2'-deoxyuridine | 95% | 396元/1g; 1320元/5g; 2376元/10g; 5016元/25g; | 展开 |
基本信息 | CAS Number | 59-14-3 | | MDL Number | MFCD00006529 | | Melting Point | 191 - 194 ℃ |
安全信息 | 图形或危害标志 |  | | 危险性说明 | H302: Harmful if swallowed.
H315: Causes skin irritation.
H319: Causes serious eye irritation.
H332: Harmful if inhaled.
H335: May cause respiratory irritation.
H340: May cause genetic defects ..
H361: Suspected of damaging fertility or the unborn child ..
| | 防范说明 | P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P233: Keep container tightly closed.
P260: Do not breathe .
P261: Avoid breathing .
P264: Wash hands thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear .
P281: Use personal protective equipment as required.
P301 + P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
P302 + P352: IF ON SKIN: Wash with plenty of soap and water.
P304: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing..
P304 + P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P308 + P313: IF exposed or concerned: Get medical advice/ attention.
P312: Call a POISON CENTER or doctor/physician if you feel unwell.
P321: Specific treatment (see relevant procedures. on this label).
P330: Rinse mouth.
P332 + P313: If skin irritation occurs: Get medical advice/ attention.
P337 + P313: If eye irritation persists: Get medical advice/attention.
P340: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P362: Take off contaminated clothing and wash before reuse.
P403: Store in a well-ventilated place.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
P405: Store locked up.
P501: Dispose of contents/container to in accordance with local regulation .
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