基本信息

技术规格

物性(参考值)

安全信息

图形或危害标志
信号词	警告
危险性说明	H315 : 造成皮肤刺激。 H319 : 造成严重眼刺激。 H413 : 可能对水生生物造成长期持续有害影响。
防范说明	P501 : 将内装物/容器送到批准的废物处理厂处理。 P273 : 避免释放到环境中。 P264 : 作业后彻底清洗皮肤。 P280 : 戴防护手套/戴防护眼罩/戴防护面具。 P302 + P352 : 如皮肤沾染：用水充分清洗。 P337 + P313 : 如仍觉眼刺激：求医/就诊。 P305 + P351 + P338 : 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P362+P364 : 脱掉沾污的衣服，清洗后方可重新使用。 P332 + P313 : 如发生皮肤刺激：求医/就诊。

相关法规

基本信息

技术规格

物性(参考值)

安全信息

图形或危害标志
信号词	警告
危险性说明	H315 : 造成皮肤刺激。 H319 : 造成严重眼刺激。 H413 : 可能对水生生物造成长期持续有害影响。
防范说明	P501 : 将内装物/容器送到批准的废物处理厂处理。 P273 : 避免释放到环境中。 P264 : 作业后彻底清洗皮肤。 P280 : 戴防护手套/戴防护眼罩/戴防护面具。 P302 + P352 : 如皮肤沾染：用水充分清洗。 P337 + P313 : 如仍觉眼刺激：求医/就诊。 P305 + P351 + P338 : 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P362+P364 : 脱掉沾污的衣服，清洗后方可重新使用。 P332 + P313 : 如发生皮肤刺激：求医/就诊。

相关法规

基本信息

安全信息

图形或危害标志
提示语	Warning
危险说明	H315 H319 H335
防范说明	P261 P305+P351+P338
WGK	3
RTECS	DV2300000
TSCA	是

应用
Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.

备注
Light and moisture sensitive. Store in a cool place. Incompatible with metals, strong acids and strong oxidizing agents.

基本信息

MDL
MFCD00007748

EINECS
259-364-6

分子式
C₁₆H₂₀N₂

分子量
240.35

熔点
168-171°

灵敏度
Moisture Sensitive. Light Sensitive. Store under Nitrogen. Ambient temperatures.

形态
Powder

溶解性
Soluble in ethyl acetate, ethanol, acetone, chloroform, toluene and dimethyl sulfoxide. Insoluble in water.

Beilstein
2808541

GHS危害和防范说明

GHS符号

Hazard Statements
H315-H319-H335
Causes skin irritation.Causes serious eye irritation.May cause respiratory irritation.

Precautionary Statements
P261-P280a-P305+P351+P338-P304+P340-P405-P501a
Avoid breathing dust/fume/gas/mist/vapours/spray.Wear protective gloves and eye/face protection.IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.Store locked up.Dispose of contents/container in accordance with local/regional/national/international regulations.

安全信息

危险类别
36/37/38
Irritating to eyes, respiratory system and skin.

安全等级
26-37-60
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.This material and its container must be disposed of as hazardous waste

RTECS
DV2300000

TSCA
是

基本信息

Ref#
32803

基本信息

Ref#
32805

MDL
7748

密度
1

产品说明

应用

3,3′,5,5′-Tetramethylbenzidine may be used as a substrate for the analysis of diclofenac in water samples and dextromethorphan and its major metabolite dextrorphan in urine samples using enzyme-linked immunosorbent assay (ELISA) and gas chromatography coupled to mass spectrometry (GC-MS).
3,3′,5,5′-四甲基联苯胺(TMB)是一种二氨基联苯胺的非致癌性取代物(Ames测试阴性)适合在ELISA实验中作为过氧化物酶底物。底物产生可溶性终产物，淡蓝色，分光光度检测在370或620-650nm可以读峰值。TMB反应能被2M H₂SO₄终止(产生黄色)，在450nm处可读。一种高敏感，特异性试剂用于检测血液，血红蛋白实验，过氧化物酶实验。

其他说明

this product will be discontinued, please find the alternative product here :
860336
用于测定商品中偶氮染料所释放的芳香胺的标准品

基本信息

产品性质

安全信息

产品说明

一般描述

本品为包含3,3′,5,5′-四甲基联苯胺(TMB)的弱酸性缓冲液。底物以单组分即用型溶液提供。速率动力学约比传统TMB配方快40％。不推荐用于需要沉淀反应产物的膜或免疫组化应用。

应用

的3,3′,5,5′-四甲基联苯胺是超灵敏的ELISA液体底物，可用以下应用的作过氧化物酶底物：

• 通过转染子测定合成的透明质酸和HAS2(透明质酸合成酶)表达
• 测定结合的α-溶血素毒素的水平
• 结合IgG成像

基本信息

产品性质

安全信息

产品说明

一般描述

四甲基联苯胺是一种过氧化物酶敏感染料。本品稳定性好，不受钙、镁离子干扰，是测定水中氯的较好方法。

包装

100 mg in glass bottle
1, 5, 25 g in glass bottle

应用

3,3′,5,5′-四甲基联苯胺(TMB)是一种二氨基联苯胺的非致癌性取代物(Ames测试阴性)适合在ELISA实验中作为过氧化物酶底物。底物产生可溶性终产物，淡蓝色，分光光度检测在370或620-650nm可以读峰值。TMB反应能被2M H₂SO₄终止(产生黄色)，在450nm处可读。一种高敏感，特异性试剂用于检测血液，血红蛋白实验，过氧化物酶实验。

基本信息

产品性质

安全信息

产品说明

应用

3,3′,5,5′-四甲基联苯胺已用于：

• Man-Rodosa-Sharpe(MRS)琼脂平皿的补充剂，在存在O2的条件下将乳杆菌菌落染成蓝色
• 用作ELISA(酶联免疫吸附测定)的底物溶液
• 定量中性粒细胞的髓过氧化物酶活性测定

包装

100 mg in glass bottle
1, 5, 100 g in glass bottle
无底玻璃瓶。内容物在插入式融锥内。

生化/生理作用

3,3′,5,5′-四甲基联苯胺/ TMB可用作色原，加速过氧化物酶反应获取产物的进程。在食品和环境净化程序中，TMB可现场监测亚氯酸基消毒剂的游离氯(FAC)。

基本信息

产品性质

安全信息

产品说明

应用

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:

• enzyme-immunoassay (EIA)
• indirect competitive enzyme-linked immunosorbent assay (ELISA)
• sandwich enzyme-linked immunosorbent assay

基本信息

产品性质

安全信息

产品说明

应用

3,3′,5,5′-四甲基联苯胺已用作竞争法和间接法酶联免疫吸附分析(ELISA)的底物。
3,3′,5,5′-四甲基联苯胺(TMB)是一种二氨基联苯胺的非致癌性取代物(Ames测试阴性)适合在ELISA实验中作为过氧化物酶底物。底物产生可溶性终产物，淡蓝色，分光光度检测在370或620-650nm可以读峰值。TMB反应能被2M H₂SO₄终止(产生黄色)，在450nm处可读。一种高敏感，特异性试剂用于检测血液，血红蛋白实验，过氧化物酶实验。

基本信息

产品性质

安全信息

产品说明

分析说明

Assay (perchloric acid titration): ≥ 99 %
Identity (IR-spectrum): conforms
Melting range: 166 - 170 °C
Loss on Drying (105°C): ≤ 0.2 %

基本信息

产品性质

安全信息

产品说明

一般描述

A soluble, non-toxic, non-flammable horseradish peroxidase substrate.

包装

1000 ml in Glass bottle
100 ml in Plastic ampoule

应用

ELISA

警告

Toxicity: Harmful (C)

外形

In a mildly acidic buffer with proprietary stabilizers, pH 3.55 ± 0.20.

其他说明

Product does not contain hazardous ingredients such as polar aprotic solvents (i.e., N,N-Dimethylformamide (DMF) or DMSO) that are found in other TMB formulations. There is also no alcohol in TMB (Soluble Substrate) so it is not flammable and does not require special handling for shipping. Unreacted TMB (Soluble Substrate) should be colorless or a very light-bluish-green prior to use.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

产品性质

安全信息

产品说明

一般描述

Peroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.
Insoluble TMB substrate supplied as a single component system. TMB is the most sensitive chromogenic substrate for detection of horseradish peroxidase (HRP)-labeled probes. In the presence of HRP and hydrogen peroxide (H₂O₂), TMB is oxidized first to a blue, cation free radical; upon further reaction with HRP/H₂O₂, or addition of acid, the radical is converted to a yellow diimine terminal oxidation product. TMB is used routinely in ELISA procedures, whereby its soluble oxidation products are measured spectrophotometrically. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate or other sugar polymers are usually added.This TMB reagent is a safe, single component system that has been developed for use in blotting procedures by a proprietary methodology that eliminates the use of sugar polymers. The reagent produces a stable, insoluble aquamarine precipitate at the reaction site with little or no background. It can significantly increase the detection limits of assays on a variety of membranes. Ideal for many immunoblotting procedures and can significantly increase detection limits of assays on a variety of membranes

包装

100 ml in Glass bottle

警告

Toxicity: Carcinogenic / Teratogenic (D)

外形

1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.

重悬

Discard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

产品性质

安全信息

产品说明

一般描述

Peroxidase substrate that produces a blue soluble end product that can be read at 370 nm or 650 nm. Use of stop solution enhances sensitivity two- to four-fold and results in a yellow solution that can be read at 450 nm.
Soluble TMB substrate supplied as a single component system.

包装

100 ml in Glass bottle

警告

Toxicity: Irritant (B)

外形

Single component system containing 1.46 mM TMB, 2.21 mM H₂O₂ in a proprietary solvent, pH 3.1 ± 0.5.

重悬

Protect from exposure to direct sunlight. Discard if the solution turns blue or turbid. The reagent is stable at room temperature, but we recommend storage in the refrigerator for longer shelf life. Warm to assay temperature before use.

其他说明

Product tested for stability at 4°C and 18-26°C. Product performance is tested on the final product by ELISA.



Suggested Procedure for Use of TMB in HRP-based ELISAs:



1. Complete all required incubations with antibodies and HRP-labeled probes.

2. Wash plate wells at least 4 times with phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), containing 0.1% Tween^®-20 detergent.

3. After the final wash, shake and blot all residual buffer from the wells.

4. Add 100 μl TMB solution to each well and incubate for 5-30 min.

5. Options for measurements:

a. For kinetic assays, the reaction can be monitored as a function of time by reading absorbance at 650 nm at intermediate intervals.

b. For endpoint assays that preserve the blue chromogen, the reaction should be stopped by addition of 100 µl of 0.1% sodium fluoride (NaF) and the absorbance read at 650 nm.

c. If increased sensitivity is desired for endpoint assays, the reaction should be stopped by addition of 100 μl of either 500 mM H₂SO₄ or 250 mM HCl and the absorbance read at 450 nm WITHIN 5 MIN. Addition of acid converts the blue radical to the yellow diimine, which absorbs at 450 nm.



NOTE: Optimal incubation times may vary depending on the amount of HRP present. If color develops too quickly, zero-order kinetics will not prevail. Dilution of the probe, antibody, or HRP-labeled reagent may be required. Variations in time, reagent volumes, and temperature may also require further standardization by the user.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

基本信息

产品性质

安全信息

产品说明

应用

BM Blue POD可溶性底物非常适用于ELISA检测。它已被用于荧光素标记的毒蜥外泌肽-4结合试验。

产品规格

分子式：C₁₆H₂₀N₂
颜色： 蓝色；反应终止后为黄色
评价方法： 光度测定法 (450 nm)

外形

即用型溶液

分析说明

吸附: 与TMB吸收光谱相同

其他说明

仅用于生命科学研究。不可用于诊断。

基本信息

产品性质

安全信息

基本信息

安全信息

图形或危害标志
提示语	Warning
危险说明	H315 H319 H335
防范说明	P261 P305+P351+P338
WGK	3
RTECS	DV2300000
TSCA	是

基本信息

安全信息

图形或危害标志
危险性说明	H315: Causes skin irritation. H319: Causes serious eye irritation. H335: May cause respiratory irritation.
防范说明	P233: Keep container tightly closed. P260: Do not breathe . P261: Avoid breathing . P264: Wash hands thoroughly after handling. P271: Use only outdoors or in a well-ventilated area. P280: Wear . P302 + P352: IF ON SKIN: Wash with plenty of soap and water. P304: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.. P304 + P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P312: Call a POISON CENTER or doctor/physician if you feel unwell. P321: Specific treatment (see relevant procedures. on this label). P332 + P313: If skin irritation occurs: Get medical advice/ attention. P337 + P313: If eye irritation persists: Get medical advice/attention. P340: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P362: Take off contaminated clothing and wash before reuse. P403: Store in a well-ventilated place. P403 + P233: Store in a well-ventilated place. Keep container tightly closed. P405: Store locked up. P501: Dispose of contents/container to in accordance with local regulation .