产品说明

一般描述

谷氨酸是非必需氨基酸。是主要的兴奋性神经递质。在重大创伤、重大手术、败血症和骨髓移植中，谷氨酸起必需氨基酸的作用。

应用

从链霉菌( Streptomyces )属提取的 L-谷氨酸氧化酶用于确定固定化谷氨酸氧化酶(GOx)的酶活性。也作为随机光学重建显微镜(STORM)成像缓冲液成分。

生化/生理作用

谷氨酸合成或降解中的主要酶；将氨基转移至α-酮戊二酸形成 L-谷氨酸或脱酰胺基 L-谷氨酸。
L-谷氨酸氧化酶催化 L-谷氨酸转化为 2-酮戊二酸。

单位定义

在 30℃、pH 7.4 条件下 1 个单位 α-酮戊二酸的生成速率为 1.0 μmol/min。

基本信息

产品性质

安全信息

产品说明

一般描述

This product has a 6xHistidine tag with a thrombin cleavage site.
L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.

应用

L-Glutamate Oxidase from Streptomyces sp may be used:

• as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
• as a component of pencil graphite electrode for biosensing L-glutamate

生化/生理作用

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.

单位定义

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

基本信息

产品性质

安全信息