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CAS: 25535-16-4
分子式: C27H34N4·2 I
分子量: 668.39
熔点: 220-225℃
中文名称: 
碘化丙啶
英文名称: 
Propidium iodide
3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenyl-phenanthridiniu dii
3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridinium diiod
3,8-diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodi
propidium diiodide
推荐供应商
货号品牌产品名称规格包装、参考价格详情
P796851MACKLIN碘化丙啶10mM in DMSO499元/1ml;  
储存:-20°C
状态:液体
P816509MACKLIN碘化丙啶生物技术级115元/5mg;   299元/25mg;   823元/100mg;   2886元/500mg;   5355元/1g;  
储存:2~8°C
状态:微红色到非常暗红色粉末
P799271MACKLIN碘化丙啶≥98%(HPLC)257元/5mg;   675元/25mg;   1798元/100mg;   3595元/250mg;  
储存:-20°C
状态:微红色到红色到非常暗红色粉末
279945J&KPropidium iodide95%134元/5MG;   488元/25MG;   1164元/100MG;  

基本信息

分子式C27H34I2N4
分子量668.39
熔点220-225
MDL编码MFCD00011921
Beilstein3843838
EINECS 编号247-081-0
存储条件Store at 2-8℃

荧光性质

λex 482 nm; λem 608 nm (pH 7.2)

λex 540 nm; λem 608 nm (bound to DNA)

一般描述

PI可用于选择性标记死细胞。

碘化丙啶是用于染色的荧光嵌入剂。它起到细胞凋亡标记的作用,具有线性DNA结合能力。碘化丙啶是组织损伤的有效指示剂。

应用

在细胞周期分析中对DNA进行染色;对细胞和细胞核进行染色;用于制备碘化丙啶(PI)储备溶液;对细胞进行染色用于流式细胞术。

安全信息

图形或危害标志
提示语Warning
危险说明H315
H319
H335
防范说明P261
P305+P351+P338
WGK3
RTECSSF7949600
931006J&KPI solution, a red-fluorescent dye that can be used to stain cells是可用于细胞染色的红色荧光染料836元/10ML;  

基本信息

分子式C27H34I2N4
分子量668.39
MDL编码MFCD00011921
Beilstein 3843838
EINECS 编号 247-081-0
存储条件Freezer -20℃

一般描述

碘化丙锭溶液用于评估神经元死亡。

应用

染色DNA;通过活力染色法研究胰岛活力;可视化间充质干细胞片中的F-肌动蛋白和细胞核;膜渗透,由汞或氙弧灯或氩离子激光激发的苯甲酸盐嵌入染料。适用于荧光显微镜、激光共聚焦扫描显微镜、流式细胞仪、荧光仪等。 用作核染色剂。

安全信息

图形或危害标志
提示语Warning
危险说明H315
H319
H335
H341
防范说明P201
P280
P305+P351+P338
P308+P313
P337+P313
WGK3
RTECSSF7949600
J66764Alfa AesarPropidium iodide3507元/100mg;  

应用
Propidium iodide is a fluorescent stain for nucleic acids. Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are early in the apoptosis process.

备注
Light Sensitive & Hygroscopic. Store in cool, dry conditions in well sealed containers. Keep container tightly closed.

基本信息

MDL
MFCD00011921

EINECS
247-081-0

分子式
C27H34I2N4

分子量
668.39

熔点
220-225°

灵敏度
Hygroscopic. Light Sensitive. Store under Argon. Ambient temperatures.

形态
Solid

溶解性
Soluble in water at 1mg/ml

Beilstein
3843838

GHS危害和防范说明

GHS符号

Hazard Statements
H301-H332-H315-H319-H335
Toxic if swallowed.Harmful if inhaled.Causes skin irritation.Causes serious eye irritation.May cause respiratory irritation.

Precautionary Statements
P261-P280a-P301+P310a-P305+P351+P338-P405-P501a
Avoid breathing dust/fume/gas/mist/vapours/spray.Wear protective gloves and eye/face protection.Wear protective gloves and eye/face protection.IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.Store locked up.Dispose of contents/container in accordance with local/regional/national/international regulations.

安全信息

危险类别
20/22-36/37/38
Harmful by inhalation and if swallowed.Irritating to eyes, respiratory system and skin.

安全等级
9-26-36/37-60
Keep container in a well-ventilated place.In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.Wear suitable protective clothing and gloves.This material and its container must be disposed of as hazardous waste

欧盟危险品标志

RTECS
SF7949600

TSCA

537059Sigma-AldrichPropidium Iodide-CAS 25535-16-4-CalbiochemMembrane impermeable DNA intercalator.1446.4元/50 MG;   2938元/100 MG;   6809.38元/250 MG;   21470元/1 G;  

产品说明

一般描述

Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.

包装

1 g in Plastic ampoule
250 mg in Plastic ampoule
50, 100 mg in Glass bottle

生化/生理作用

Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Cell permeable: no

警告

Toxicity: Irritant (B)

制备说明

Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他说明

Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C27H34I2N4
分子量668.39
MDL编号MFCD00011921

产品性质

质量水平100
形式solid
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
protect from light
颜色dark red
inputsample type intact cells
荧光λex ~536 nm
λem ~617 nm
运输ambient
储存温度2-8℃
InChI1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI keyXJMOSONTPMZWPB-UHFFFAOYSA-M

安全信息

象形图GHS08
警示用语:Warning
危险声明H341
预防措施声明P201 - P202 - P280 - P308 + P313 - P405 - P501
危险分类Muta. 2
储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
P4170Sigma-AldrichPropidium iodide≥94.0% (HPLC)365.68元/10 MG;   730.08元/25 MG;   2085.96元/100 MG;   2490.59元/250 MG;   4543.87元/500 MG;   8828.89元/1 G;  

产品说明

应用

用于核酸的荧光染色。 细胞膜的完整性可将阻止活细胞和凋亡细胞的碘化丙锭染色。 当与可对活细胞或处在凋亡过程早期细胞进行染色的其他染料一起使用时,碘化丙锭可用于流式细胞术以对细胞活力进行评估。

包装

10, 25, 100, 250, 500 mg in glass bottle
1 g in glass bottle

特点和优势

该化合物是细胞凋亡研究的特色产品。点击此处以了解更多的细胞凋亡特色产品。可访问sigma.com/discover-bsm了解更多关于用于其他研究领域生物活性小分子。

基本信息

经验(实验)分子式C27H34I2N4
分子量668.39
Beilstein3843838
EC 号247-081-0
MDL编号MFCD00011921
PubChem化学物质编号57654524
NACRESNA.77

产品性质

质量水平200
测定≥94.0% (HPLC)
mp220-225 ℃ (dec.) (lit.)
储存温度2-8℃
SMILES string[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI keyXJMOSONTPMZWPB-UHFFFAOYSA-M

安全信息

象形图GHS08
警示用语:Warning
危险声明H341
预防措施声明P201 - P308 + P313
危险分类Muta. 2
储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备dust mask type N95 (US), Eyeshields, Gloves
79214Sigma-AldrichBacteria stain propidium iodide solution1 mg/mL in H2O, ready to use solution, 100 assays1552.44元/4 X 25 μL;  

产品说明

应用

膜渗透,由汞或氙弧灯或氩离子激光激发的苯甲酸盐嵌入染料。适用于荧光显微镜、激光共聚焦扫描显微镜、流式细胞仪、荧光仪等。 用作核染色剂。

基本信息

经验(实验)分子式C27H34I2N4
分子量668.39
Beilstein3843838
MDL编号MFCD00011921
PubChem化学物质编号329768314
NACRESNA.32

产品性质

质量水平100
测定≥90.0% (HPLC)
形式liquid
质量ready to use solution, 100 assays
浓度1 mg/mL in H2O
荧光λex 530 nm; λem 620 nm±5 nm in 0.1 M phosphate pH 7.0
储存温度−20℃
SMILES string[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI keyXJMOSONTPMZWPB-UHFFFAOYSA-M

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable
537060Sigma-AldrichPropidium Iodide Solution-CAS 25535-16-4-CalbiochemA convenient form of propidium iodide useful for flow cytometry studies.1638.5元/5 ML;  

产品说明

一般描述

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

包装

5 ml in Plastic ampoule

生化/生理作用

Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Cell permeable: no

警告

Toxicity: Standard Handling (A)

外形

50 µg/ml in PBS, pH 7.4.

重悬

Following initial use, aliquot and freeze (-20°C).

其他说明

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C27H34I2N4
分子量668.39
MDL编号MFCD00011921

产品性质

质量水平100
形式liquid
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
protect from light
inputsample type intact cells
荧光λex 536 nm
λem 617 nm
运输ambient
储存温度2-8℃
InChI1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI keyXJMOSONTPMZWPB-UHFFFAOYSA-M

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable
P4864Sigma-AldrichPropidium iodide solutionsolution (1.0 mg/ml in water)1173.23元/10 ML;  

产品说明

一般描述

碘化丙锭溶液用于评估神经元死亡。

应用

碘化丙啶溶液已用于:

  • 染色DNA
  • 通过活力染色法研究胰岛活力
  • 可视化间充质干细胞片中的F-肌动蛋白和细胞核


膜渗透,由汞或氙弧灯或氩离子激光激发的苯甲酸盐嵌入染料。适用于荧光显微镜、激光共聚焦扫描显微镜、流式细胞仪、荧光仪等。 用作核染色剂。

包装

10 mL in glass bottle

基本信息

经验(实验)分子式C27H34I2N4
分子量668.39
Beilstein3843838
MDL编号MFCD00011921
PubChem化学物质编号24898563
NACRESNA.47

产品性质

测定≥94%
质量水平200
形式solution (1.0 mg/ml in water)
溶解性water: 1 mg/mL
εmax5400-6400 at 491-495 nm in phosphate buffer
application(s)diagnostic assay manufacturing
hematology
histology
储存温度2-8℃
SMILES string[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI keyXJMOSONTPMZWPB-UHFFFAOYSA-M

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves
81845Sigma-AldrichPropidium iodide≥94% (HPLC)753.97元/25 MG;   1941.45元/100 MG;  

产品说明

一般描述

PI可用于选择性标记死细胞。
碘化丙啶是用于染色的荧光嵌入剂。它起到细胞凋亡标记的作用,具有线性DNA结合能力。碘化丙啶是组织损伤的有效指示剂。

应用

碘化丙啶被用于:

  • 在细胞周期分析中对DNA进行染色
  • 对细胞和细胞核进行染色
  • 用于制备碘化丙啶(PI)储备溶液
  • 对细胞进行染色用于流式细胞术


用于划痕负载染料迁移试验中

包装

25, 100 mg in glass bottle
无底玻璃瓶。内容物在嵌入的融合锥中。

特点和优势

该化合物是细胞凋亡研究的推荐产品。点击此处,了解更多精选的细胞凋亡产品。了解更多有关用于其他研究领域的生物活性小分子的信息,请访问sigma.com/discover-bsm。

基本信息

经验(实验)分子式C27H34I2N4
分子量668.39
Beilstein3843838
EC 号247-081-0
MDL编号MFCD00011921
PubChem化学物质编号329769221
NACRESNA.77

产品性质

质量水平200
测定≥94% (HPLC)
mp220-225 ℃ (dec.) (lit.)
荧光λex 482 nm; λem 608 nm (pH 7.2)
λex 540 nm; λem 608 nm (bound to DNA)
储存温度2-8℃
SMILES string[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI keyXJMOSONTPMZWPB-UHFFFAOYSA-M

安全信息

象形图GHS08
警示用语:Warning
危险声明H341
预防措施声明P201 - P202 - P280 - P308 + P313 - P405 - P501
危险分类Muta. 2
储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备dust mask type N95 (US), Eyeshields, Gloves
CAS号首数字顺序排列: 1 2 3 4 5 6 7 8 9