基本信息

技术规格

物性(参考值)

安全信息

图形或危害标志
信号词	警告
危险性说明	H315 : 造成皮肤刺激。 H319 : 造成严重眼刺激。
防范说明	P264 : 作业后彻底清洗皮肤。 P280 : 戴防护手套/戴防护眼罩/戴防护面具。 P302 + P352 : 如皮肤沾染：用水充分清洗。 P337 + P313 : 如仍觉眼刺激：求医/就诊。 P305 + P351 + P338 : 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P362+P364 : 脱掉沾污的衣服，清洗后方可重新使用。 P332 + P313 : 如发生皮肤刺激：求医/就诊。

相关法规

基本信息

产品描述

Caffeic Acid Phenethyl Ester是强效的特异性NF-κB活化抑制剂，也能够表现出抗氧化、免疫调节和抗炎活动。

靶点（IC50 & Targe）

NF-κB

体外研究

Caffeic acid phenethyl ester能够通过抑制p65的入核转运来抑制佛波酯，神经酰胺，强心酸过氧化氢等诱导的NF-kB的激活。[1] 在一系列的肿瘤细胞系中，Caffeic acid phenethyl ester具有抗增殖的活性，其对26-L5, B16-BL6恶性瘤, 人类HT-1080的 EC50 分别是 1.76, 3.16, 13.7,和44.0 μM。[2] Caffeic acid phenethyl ester通过抑制ROS的形成和抑制半胱氨酸天冬氨酸蛋白酶的活性发挥抗凋亡的作用。Caffeic acid phenethyl ester通过抑制NF-kB信号通路来减弱LPS诱导的炎症和LPS诱导的HSCs细胞对细胞因子的敏感性。[4]

体内研究

C57BL/6 and BALB/c小鼠腹腔内注射10毫克/千克Caffeic acid phenethyl ester能够抑制在路易斯肺细胞癌，结肠癌，和黑色素瘤的生长和肿瘤的血管新生。[5]在动物体内(5, 10, 20 mg/kg) 的Caffeic acid phenethyl ester通过减少胸腺的重量和胸腺和脾的孔性而具有免疫调节的作用。[6]

细胞实验

Cell lines: 小鼠结肠 26-L5, 小鼠B16-BL6黑色素瘤,人类HT-1080 纤维肉瘤和人类肺 A549 腺癌细胞系。

Concentrations: ～200 μM

Incubation Time: 3天

Method:人HT-1080纤维肉瘤，人肺癌A549腺癌细胞和鼠B16-BL6黑色素瘤细胞在含有10%的血清，0.1%碳酸盐，和2mM的谷氨酰胺的EMEM培养基中培养。鼠结肠癌细胞 B16-BL6用含有相同添加成分的RPMI中培养。除了A-549以外，这些都是有高度侵染能力的细胞系。细胞存活实验采用标准的MTT方法。简单的说，对数生长的细胞收集以后以2000个每孔的密度种植于96孔板。24小时孵育后，用含有不同浓度的样品的100ul培养基处理细胞，5%的二氧化碳37度条件下培养72小时。加入MTT3小时以后用Perkin Elmer HTS-7000测定550纳米波长的吸收值。待测样品先用DMSO溶解，再用培养基稀释。DMSO的终浓度小于0.25%。对照组还有相同浓度的DMSO。5-氟尿嘧啶(5-FU) 和 doxorubicin HCl用作阳性对照。EC50值通过取4个孔数据的平均值来计算的。

(Only for Reference)

动物实验

Animal Models: 接种Lewis肺肿瘤，结肠癌以及黑色素瘤细胞的C57BL/6 和 BALB/c 小鼠

Formulation: PBS

Dosages: ～10毫克/千克

Administration: 腹腔注射

(Only for Reference)

参考文献

[1] Natarajan K, et al. Proc Natl Acad Sci U S A. 1996, 93(17), 9090-9095.

[2] Banskota AH, et al. J Ethnopharmacol. 2002, 80(1), 67-73.

[3] Amodio R, et al. Int J Dev Neurosci. 2003, 21(7), 379-389.

[4] Zhao WX, et al. Int J Mol Med. 2014, 33(3), 687-694.

[5] Chung TW, et al. J Mol Med (Berl). 2013, 91(2), 271-282.

[6] Park JH, et al. Int Immunopharmacol. 2004, 4(3), 429-436.

安全信息

图形或危害标志
提示语	Warning
危险说明	H315 H319 H335
防范说明	P261 P305+P351+P338
WGK	3
RTECS	UD3334375

产品说明

应用

咖啡酸苯乙酯(CAPE)已用于测定CAPE对甲亢的作用，作为培养基组分检测乳腺癌细胞系MDA-MB-231的细胞毒性，研究其对大鼠胺碘酮诱导肺纤维化的影响。

生化/生理作用

CAPE具有多种特性，如抗癌、免疫调节活性、抗氧化、抗病毒、抗炎、降血糖作用、心脏保护功能以及胃分泌抑制作用。它还对一种乳腺癌肿瘤生长因子——成纤维细胞生长因子2(FGF-2)具有抑制作用。
咖啡酸苯乙酯是核转录因子 NF-κ B的特异性抑制剂。

特点和优势

该化合物是基因调控研究的特色产品。 点击此处 ，发现更多特色基因调控产品。在 sigma.com/discover-bsm 了解更多关于其他研究领域的生物活性小分子。

基本信息

产品性质

安全信息

产品说明

一般描述

A cell-permeable active component of propolis from honeybee hives. Known to have antiviral, anti-inflammatory, and immunomodulatory properties. Has also recently been shown to act as a potent and specific inhibitor of NF-κB activation. An inhibitor of HIV-1 integrase activity. Inhibits the growth of different types of transformed cells. Has been reported to suppress lipid peroxidation. Inhibits ornithine decarboxylase, protein tyrosine kinase (PTK), and lipoxygenase activities. Also induces apoptosis in cloned rat embryo fibroblast (CREF) cells transformed by a number of different oncogenes.
A cell-permeable active component of propolis from honeybee hives. Known to have antiviral, anti-inflammatory, and immunomodulatory properties. An inhibitor of HIV-1 integrase activity. Inhibits the growth of different types of transformed cells. Has been reported to suppress lipid peroxidation. Inhibits ornithine decarboxylase, protein tyrosine kinase (PTK) and lipoxygenase activities. Also induces apoptosis in cloned rat embryo fibroblast (CREF) cells transformed by a number of different oncogenes. Has also recently been shown to act as a potent and specific inhibitor of NF-κB activation.

包装

25 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Cell permeable: yes
Primary Target
HIV-integrase
Product does not compete with ATP.
Reversible: no

警告

Toxicity: Irritant (B)

重悬

Following reconstitution aliquot and freeze at -20°C. Stock solutions are stable for up to 3 months at -20°C.

其他说明

Nicklaus, M.C., et al. 1997. J. Med. Chem.40, 920.
Natarajan, K., et al. 1996. Proc. Natl. Acad. Sci. USA93, 9090.
Burke, T.R., Jr., et al. 1995. J. Med. Chem. 38, 4171.
Laranjinha, J., et al. 1995. Arch. Biochem. Biophys. 323, 373.
Su, Z.-Z., et al. 1995. Anticancer Res. 15, 1841.
Zheng, Z.S., et al. 1995. Oncol. Res. 7, 445.
Su, Z.-Z., et al. 1994. Cancer Res. 54, 1865.
Fesen, M.R., et al. 1993. Proc. Natl. Acad. Sci. USA90, 2399.
Guarini, L., et al. 1992. Cell. Mol. Biol. 38, 513.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

产品性质

安全信息

基本信息

安全信息

图形或危害标志
危险性说明	H315: Causes skin irritation. H319: Causes serious eye irritation. H335: May cause respiratory irritation.
防范说明	P233: Keep container tightly closed. P260: Do not breathe . P261: Avoid breathing . P264: Wash hands thoroughly after handling. P271: Use only outdoors or in a well-ventilated area. P280: Wear . P302 + P352: IF ON SKIN: Wash with plenty of soap and water. P304: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.. P304 + P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P312: Call a POISON CENTER or doctor/physician if you feel unwell. P321: Specific treatment (see relevant procedures. on this label). P332 + P313: If skin irritation occurs: Get medical advice/ attention. P337 + P313: If eye irritation persists: Get medical advice/attention. P340: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P362: Take off contaminated clothing and wash before reuse. P403: Store in a well-ventilated place. P403 + P233: Store in a well-ventilated place. Keep container tightly closed. P405: Store locked up. P501: Dispose of contents/container to in accordance with local regulation .