生物制药 HPLC 分析->N-糖样品前处理和分析->外切糖苷酶

AdvanceBio Sialidase C，1 U. 酶从寡糖、糖蛋白、复杂碳水化合物中水解释放 α(2-3,6)-连接的唾液酸。包括 5x 反应缓冲液 B [250 mmol/L 磷酸钠 pH 6.0]。

体积	100 µL
分子量	~41,000 daltons
单位	1 U
** pH 值	6
浓度	10 U/mL
酶单位定义	One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5
酶来源	Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24. Sialidases are also known as neuraminidases.
酶比活性	≥40 units/mg
酶特异性	This enzyme releases a2-3, and a2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc a2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. Sialidase A (GK80040) is recommended for this application.
酶配方	20 mM Tris HCl pH 7.5, containing 25 mM NaCl