生物制药 HPLC 分析->N-糖样品前处理和分析->外切糖苷酶

AdvanceBio Sialidase S，组氨酸标记. 酶从寡糖、糖蛋白、复杂碳水化合物中水解释放 α(2-3)-连接的唾液酸。取代唾液酸酶 S (GK80020)。包括 5x 反应缓冲液 B [250 mmol/L 磷酸钠 pH 6.0]。

分子量	~75 kD
单位	3 U
酶单位定义	One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5
酶来源	AdvanceBio Sialidase S. Recombinant from Streptococcus pneumoniae, expressed in E. coli. 6xHis-tagged. Sialidases are also known as neuraminidases.
酶特异性	This enzyme preferentially releases a(2-3)-linked N-acetylneuraminic acid (Neu5Ac, NANA) from complex carbohydrates. Sialidase S is also active against a(2-3)-linked N-glycolyneuraminic acid [1] (Neu5Gc, NGNA), although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. Although the a(2-3)-linkage is preferred, Sialidase S (also known as NanB) is also capable of cleaving a(2-6)-linked sialic acid [3], particularly at higher concentrations. Adding an excess of this enzyme can lead to some cleavage of a(2-6)-linked sialic acid. Using GK80021 as directed in the certificate of analysis reduces the likelihood of these effects. Users may wish to optimize the enzyme concentration needed for specific proteins. May be used for exoglycosidase sequencing with Sialidase A (GK80040), which has a broader specificity, releasing a(2-3,6,8,9)-linked sialic acids. Sialidases are also known as neuraminidases.
酶配方	Supplied lyophilized. 20 mM Tris-HCl, 25 mM NaCl (pH 7.5) upon reconstitution as indicated