生物制药 HPLC 分析->N-糖样品前处理和分析->AdvanceBio Gly-X 技术

AdvanceBio Gly-X 2-AB Express 标记模块，24-ct. 利用 2-AB（2-氨基苯甲酰胺）染料对游离多聚糖进行衍生化。经验证的 2-AB 染料可确保正在进行的项目的数据连续性。

前处理时间（近似）	130 min (24 samples)
去糖基化效率	>99 %
回收率	>80 %
应用	Reductive amination 2-AB labeling chemistry without dry down
技术	LC 和 LC/MS
标签	2-AB
由用户提供的设备和试剂	Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended), ~4 mL per sample DI water, ~100 µl per sample Gly-X vacuum manifold spacer and heater block lid (included in GX400 Gly-X 2-AB Express starter kit, or use GX100 spacer and alternate heater lid) Two Thermo Scientific 88871001 Compact dry block heaters with 96 well PCR heat blocks. Two dry block heaters with 96 well PCR heat blocks. One of the heaters can be substituted with a 96-well thermocycler. Denaturation and deglycosylation can be performed using a thermocycler. A heat block is required for the labeling step. Vacuum manifold (Millipore MSVMHTS00) Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L Pump 2522)
稳定性	-20 °C 下至少 1 个月，4-8 °C 下 5 天
蛋白质上样量范围	1-40 µg
计数	24
试剂盒组成	Sample Prep Module