试剂仪器设备原料
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细胞培养基 >> 细胞株
BALB/3T3 clone A31小鼠胚胎成纤维细胞
BALB/3T3 clone A31小鼠胚胎成纤维细胞图片
产品介绍
目录号
Serial
GNM 3
标识符
Identifier
CSTR:19375.09.3101MOUGNM3
描述
Description
BALB/3T3 clone A31 是 1968年S.A. Aaronson和 G.T. Todaro从裂解的14到17天的 BALB/c 小鼠胚胎中建立的几株细胞之一(BALB/3T12)。
细胞分裂对接触抑制特别敏感,生长需高倍数稀释,饱和浓度低,对癌基因DNA病毒SV40和小鼠肉瘤病毒高度易感。
检测发现鼠痘病毒阴性。
2012年新引进。通过支原体检测。
动物种别
Organism
小鼠
性别
Gender
***
组织来源
Tissue and Cell Type
器官:胚胎品系:BALB/c细胞类型:成纤维细胞
形态
Morphology
成纤维细胞
培养基和添加剂
Complete Growth Medium and Culture Conditions
DMEM培养基(GIBCO by life technologies ,货号C11995500BT),90%;优质胎牛血清,10%。
气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCE22708: Aaronson SA, Todaro GJ. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J. Cell. Physiol. 72: 141-148, 1968. PubMed: 430100626022: Todaro GJ, Aaronson SA. Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells. Virology 38: 174-202, 1969. PubMed: 430652326023: Aaronson SA, Todaro GJ. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024-1026, 1968. PubMed: 430164726024: Jainchill JL, Todaro GJ. Stimulation of cell growth in vitro by serum with and without growth factor. Relation to contact inhibition and viral transformation. Exp. Cell Res. 59: 137-146, 1970. PubMed: 419442932554: Thompson SA, et al. COOH-terminal extended recombinant amphiregulin with bioactivity comparable with naturally derived growth factor. J. Biol. Chem. 271: 17927-17931, 1996. PubMed: 866353532907: Anderson MT, et al. Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins. Proc. Natl. Acad. Sci. USA 93: 8508-8511, 1996. PubMed: 871090092346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.
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