试剂仪器设备原料
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细胞培养基 >> 细胞株
AB2.2小鼠胚胎干细胞
AB2.2小鼠胚胎干细胞图片
产品介绍
目录号
Serial
SCSP-217
标识符
Identifier
CSTR:19375.09.3101MOUSCSP217
描述
Description
该细胞系已广泛用于创建基因敲除小鼠。HPRT负突变使该品系可用于染色体工程。已经表明该小鼠ES细胞系具有生殖能力。每个批次均通过本库支原体检测,结果为阴性。我库冻存时为P24代。
培养时需使用CF-1 MEF(SCSP-105R)作为饲养层细胞。
动物种别
Organism
小鼠,129S5/SvEvBrd(原129/SvEvBrd)品系
性别
Gender
雄性
组织来源
Tissue and Cell Type
小鼠胚胎内细胞团
形态
Morphology
球形克隆
培养基和添加剂
Complete Growth Medium and Culture Conditions
mES完全培养液配方(600 ml):
DMEM (Invitrogen 12430)         497 ml
ES级FBS (biochrom S4115)       90 ml
Glutamax (Invitrogen 35050)        6 ml
NEAA (Invitrogen 11140)          6 ml
LIF (Millipore ESG1107)          60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985)          1.5 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCEBader A, Al-Dubai H, Weitzer G. Leukemia Inhibitory Factor Modulates Cardiogenesis in Embryoid Bodies in Opposite Fashions. Circ. Res. 86: 787-794, 2000. PubMed: 10764413 Wolcik SM, Longley MA, Roop DR. Discovery of a novel murine keratin 6 (K6) isoform explains the absence of hair and nail defects in mice deficient for K6a and K6b. J. Cell Biol. 154: 619-630, 2001. PubMed: 11489919 Liu Y, et al. Sox17 is essential for the specification of cardiac mesoderm in embryonic stem cells. Proc Natl Acad Sci USA. 104(10): 3859-3864, 2007. PubMed: 17360443 Adams DJ, et al. A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction. Genomics 86(6): 753-758. 2005. PubMed: 16257172Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999.PubMed: 10430671 Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391 Bradley A, Zheng B, Liu P. Thirteen years of manipulating the mouse genome: a personal history. Int. J. Dev. Biol. 42 (7): 943-950, 1998. PubMed: 9853825
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