产品介绍 目录号
Serial | SCSP-571 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP571 | 描述
Description | 人膀胱癌细胞。该患者在切除肿瘤之前有4个月的血尿病史。后来发现了向骨髓的转移。超微结构研究表明存在微绒毛和脂质体，但未观察到桥粒。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X; CSF1PO: 10; D13S317: 11,14; D16S539: 9,11; D5S818: 12; D7S820: 8,9; THO1: 6,9.3; TPOX: 8; vWA: 14,16 | 动物种别
Organism | 人 | 性别
Gender | 女性，67岁 | 组织来源
Tissue and Cell Type | 膀胱 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人膀胱癌细胞 TCCSUP 完全培养液 配方(100 ml)
EMEM (Invitrogen，11090081) 87 ml
FBS (Gibco)10 ml
Glutamax(invitrogen 35050061) 1 ml
Non-essential Amino Acids, 100x (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml
参考传代周期：6-8天1次
参考传代比例：1:2-1:3
参考换液频率：每周2-3次
冻存液配方：完全培养液95%，DMSO 5%
细胞状态：贴壁生长
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756 |
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