产品说明

一般描述

牛乳脂蛋白脂酶(LPL)是一种糖蛋白。它以同型二聚体形式存在，由两个N-连接的寡糖组成。具有热不稳定性。

应用

牛乳脂蛋白脂酶已用于：

• 研究脂蛋白脂酶作为补充剂对乳腺癌细胞MDA-MB-231中DiI(1,1′-双十八烷基-3,3,3′-四甲基吲哚碳菁高氯酸盐)-极低密度脂蛋白(VLDL)摄取的影响
• 处理人脑微血管内皮细胞(HBMEC)以脂解富含三酰甘油的脂蛋白(TGRL)
• 检测脂蛋白脂酶对正常人星形胶质细胞基因表达的影响

包装

5000 units in glass bottle

生化/生理作用

牛乳脂蛋白脂酶(LPL)具有最高的脂解活性。它与酪蛋白胶束有关。LPL调节甘油三酯利用度，具有位点特异性。脂酶通常催化甘油三酯的脂裂解，尤其是甘油三酯sn-1和sn-3位的脂肪酸。

单位定义

以 p -硝基苯丁酸酯为底物，在 37℃、pH 7.2 条件下，1 个单位每分钟将释放 1.0 nmol p -硝基苯酚。

外形

用 3.8 M 硫酸铵、0.02 MTris HCl (pH 8.0) 混悬

制备说明

亲和纯化

基本信息

产品性质

安全信息

产品说明

一般描述

脂肪酶是将三酰基甘油酯分解成游离脂肪酸和甘油的水解酶。

应用

当与 L-α-甘油磷酸氧化酶(G3O-301、G3O-311、G3O-321)和甘油激酶(GYK-301、GYK-311)偶联时，该酶可用于酶促测定血清中的甘油三酯。通常，在 37^oC 下，反应可在 5 分钟内完成，每次试验使用 2.5 ^oC~3.0 单位的酶(3.0ml)，pH 约为 7.0。来源于假单胞菌属的脂肪酶已被用于评估微波辐射辅助棕榈油酶促合成生物柴油的研究。

包装

500, 1000 units in poly bottle

生化/生理作用

来源于假单胞菌的脂肪酶显示出抑制人外周血中单核细胞的趋化性。
可水解甘油三酯、甘油二酯、甘油单酯(速率依次递减)。

物理属性

等电点：5.95-/+0.05
抑制剂：Hg^++、 Ag⁺、离子型去垢剂
最佳 pH 值：7.0-9.0
最佳温度：45-50^oC
pH 稳定性：pH 7.0 - 9.0 (25^oC, 20 小时)
热稳定性：低于 55^oC(pH 7.0，10 分钟)

单位定义

在 pH7.0、37℃、牛血清白蛋白存在下，一单位酶每分钟从甘油三酯中产生 1.0 μmole 甘油。

外形

冻干品，含有 Mg⁺²、胆酸钠和牛血清白蛋白(作为稳定剂)

分析说明

缩二脲法测定蛋白

基本信息

产品性质

安全信息

产品说明

生化/生理作用

Lipoprotein lipase belongs to the family of triglyceride lipases. It hydrolyses triglycerides in triglyceride-rich ApoB-containing lipoproteins.

单位定义

1 U corresponds to the amount of enzyme which liberates 1 μmol of oleic acid per minute at pH 8.0 and 37℃ (cholesteryl oleate, Cat. No. 26850, as substrate)

基本信息

产品性质

安全信息

产品说明

一般描述

Lipoprotein Lipase (LPL) is a glycerol ester hydrolase. Several bacteria that produce LPL, belongs to the genus Pseudomonas, Serratia and Mucor.
Lipoprotein lipase hydrolyzes triglycerides in plasma lipoproteins causing release of fatty acids for metabolic purposes in muscles and adipose tissue.

应用

Lipoprotein Lipase from Burkholderia sp. has been used in the subcellular fractionation of mussel Mytilus galloprovincialis to identify the biomolecule attached to cytosolic fraction of okodaic acid. It has also been used to test its effect on serum amyloid A induced granulocyte colony-stimulated factor (G-CSF) expression in response to bacterial lipoprotein.
Lipoprotein lipase has been used in a study to assess the role of lipogenic enzymes in colorectal cancer. It has also been used in a study to investigate lipasemic activity of low molecular weight heparin in rats.

包装

10 mg in glass bottle
50 mg in poly bottle

生化/生理作用

Lipoprotein Lipase (LPL) hydrolysis triacylglycerol moieties in chylomicron and low density lipoproteins. LPL attaches lipoprotein to the vessel wall and facilitates their uptake. Abnormalities in LPL is associated with Alzheimer′s disease, atherosclerosis, obesity and chylomicronaemia.
Due to its lipolytic activity, lipoprotein lipase was shown to effectively block the spread of hepatitis C virus into healthy cells.
Lipoprotein lipase belongs to the family of triglyceride lipases. It hydrolyses triglycerides in triglyceride-rich ApoB-containing lipoproteins.

单位定义

One unit will release 1.0 nmole of p-nitrophenol per min at pH 7.2 at 37 ℃ using p-nitrophenyl butyrate as substrate.

基本信息

产品性质

安全信息

产品说明

一般描述

Lipoprotein Lipase (LPL) is majorly secreted by myocytes and adipocytes in humans and is crucial for triglyceride homeostatis. Mutations in the catalytic domain of LPL impairs its interaction with glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1 (GPIHBP1). The N-terminal catalytic domain is essential for lipolysis. The C-terminal is crucial for binding lipoproteins. Altered LPL levels may play role in the pathogenesis of atherosclerosis, coronary heart disease and chronic lymphocytic leukemia.

应用

Lipoprotein Lipase from Pseudomonas sp. has been used in the enzymatic erosion studies in gamma irradiated poly(trimethylene carbonate) (PTMC) films.

生化/生理作用

Malic dehydrogenase catalyzes the dehydrogenation of L-malate by NAD+.
Lipoprotein lipase belongs to the family of triglyceride lipases. It hydrolyses triglycerides in triglyceride-rich ApoB-containing lipoproteins.

单位定义

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40℃ (triolein, Cat. No. 62314 as substrate)

其他说明

Preparation of aldol acceptors (R)- and (S)-3-azido-2-hydroxypropanal via lipase-catalyzed resolution of the racemic acetal precursor; Effect of enzyme form on its properties in toluene

基本信息

产品性质

安全信息