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CAS: 9002-08-8
英文名称: 
trypsinogen
trypsinogen from bovine pancreas
推荐供应商
货号品牌产品名称规格包装、参考价格详情
T984946MACKLIN胰蛋白酶原≥10,000 BAEE units/mg protein5100元/250mg;   展开
储存:-20°C, 密封, 干燥
状态:米色粉末
T9011Sigma-AldrichTrypsinogen, PMSF treated from bovine pancreasFor use as a marker in SDS-PAGE6000.59元/1 VIAL;   展开

产品说明

生化/生理作用

Hereditary pancreatitis was shown to be caused by a Arg-His substitution at residue 117 of trypsinogen causing auto-activation of trypsinogen to trypsin.

基本信息

NACRESNA.56

产品性质

质量水平200
生物来源bovine pancreas
形式powder
分子量 ~24 kDa
包装vial of 25 mg
application(s)detection
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)
T1143Sigma-AldrichTrypsinogen from bovine pancreasessentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin)2380.16元/250 MG;   7849.85元/1 G;   展开

产品说明

一般描述

Trypsinogen is a proenzyme (zymogen) that is activated to form trypsin. It is synthesized in the pancreas and activated by enterokinase once it reaches the lumen of the small intestine. Bovine trypsinogen is a single polypeptide chain of 229 amino acids that is cross linked by six disulfide bridges. Enterokinase cleaves a hexapeptide to from the NH2 terminus of trypsinogen at the Lys6 - Ile7 peptide bond and activates it. Trypsin, thus formed, autocatalytically activates more trypsinogen to trypsin. This native form of trypsin is called β-trypsin, which undergoes autolysis at Lys131 - Ser132 resulting in α-trypsin that is held together by disulfide bridges. Trypsin is a serine protease with His46 and Ser183 at the active site. The pH optimum of trypsin is 7 - 9.

应用

Trypsinogen from bovine pancreas is suitable for use in:

  • the secondary structure analysis of proteins in H2O solution using single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy
  • tuning and calibration of electrospray ionization quadrupole time-of-flight (ESI-Q-TOF) mass spectrometer
  • the secondary structure analysis of proteins by infrared (IR) spectroscopy
  • SDS-PAGE as a molecular weight standard (24kDa)

包装

1 g in glass bottle
250 mg in glass bottle

生化/生理作用

Phytic acid complexed with calcium has been shown to increase the secretion of trypsinogen unable to be cleaved for activation. It also reduced the stabilization effect of calcium on activated trypsin. The active form of trypsinogen, referred to as trypsin, cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. It also hydrolyzes ester and amide linkages of synthetic derivatives of amino acids such as, benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl-L-arginine methyl ester (TAME), etc.

单位定义

One BAEE unit is equal to a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 ℃ and a reaction volume of 3.2 mL (1 cm light path).

基本信息

EC 号232-651-3
NACRESNA.56

产品性质

质量水平200
生物来源bovine pancreas
测定85-100% (UV)
形式essentially salt-free, lyophilized powder
specific activity≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin)
分子量 23,981 Da by calculation
technique(s)mass spectrometry (MS): suitable
溶解性H2O: soluble 10 mg/mL
UniProt登记号Q29463
储存温度−20℃
Gene Informationbovine ... TRYP8(282603)

安全信息

象形图GHS08
警示用语:Danger
危险声明H315 - H319 - H334
预防措施声明P302 + P352 - P305 + P351 + P338
危险分类Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Faceshields, Gloves, type N95 (US)
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