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CAS: 69-78-3
分子式: C14H8N2O8S2
分子量: 396.34
熔点: 240-245℃
中文名称: 
5,5'-二硫代-双(硝基苯甲酸)
DTNB,5,5-二硫基-双(2-硝基苯甲酸)
5,5'-二硫双(2-硝基苯甲酸)
5,5’-二硫双(2-硝基苯甲酸)
二硫代硝基苯甲酸
英文名称: 
3,3'-dithiobis(6-nitro-benzoic aci
2,2'-dinitro-5,5'-dithiodibenzoesaeure
2,2'-dinitro-5,5'-dithiodibenzoic acid
3,3'-dithiobis(6-nitrobenzoic acid)
3,3'-dithiobis[6-nitro-benzoic aci
dithionitrobenzoic acid
dtnb
5,5'-dithiobis(2-nitrobenzoic acid)
5,5-Dithiobis(2-nitrobenzoic acid)
5,5'-dithiobis(2-nitrobenzoic
5,5'-dithiobis(2-nitrobenzoicacid)
3-Carboxy-4-nitrophenyl disulfide
Bis(3-carboxy-4-nitrophenyl)disulfide
Ellman's Reagent
展开更多英文名
推荐供应商
货号品牌产品名称规格包装、参考价格详情
D890349MACKLIN5,5'二硫代双(2-硝基苯甲酸)99%, suitable for determination of sulfhydryl groups98元/1g;   336元/5g;   1056元/25g;   3198元/100g;   展开
储存:室温
状态:淡-黄色固体
D806870MACKLIN5,5'二硫代双(2-硝基苯甲酸)98%39元/1g;   124元/5g;   408元/25g;   1602元/100g;   展开
储存:室温
状态:黄色固体
767035MACKLIN5,5'二硫代双(2-硝基苯甲酸)10mM in DMSO186元/1ml;   展开
储存:-20°C
D0944TCI5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]>98.0%(T)(HPLC)305元/1G;   855元/5G;   2900元/25G;   展开

基本信息

纯度/分析方法>98.0%(T)(HPLC)
分子式/分子量C14H8N2O8S2 = 396.34
外观与形状(20℃)固体
包装和容器 1G-带有塑料内管的玻璃瓶 (查看图片)
Reaxys-RN1896821
PubChem物质ID87567535
SDBS (AIST Spectral DB)460
Merck Index (14)3551
MDL编号MFCD00007140

技术规格

AppearanceLight yellow to Yellow powder to crystal
Purity(HPLC)min. 98.0 area%
Purity(Neutralization titration)min. 98.0 %
Absorbance(E1%1cm)max. 5.0(412.0 nm, buffer sol. pH7.0 phosphate)

物性(参考值)

熔点238 ℃

安全信息

图形或危害标志
信号词警告
危险性说明H315 : 造成皮肤刺激。
H319 : 造成严重眼刺激。
防范说明P264 : 作业后彻底清洗皮肤。
P280 : 戴防护手套/戴防护眼罩/戴防护面具。
P302 + P352 : 如皮肤沾染:用水充分清洗。
P337 + P313 : 如仍觉眼刺激:求医/就诊。
P305 + P351 + P338 : 如进入眼睛:用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出,取出隐形眼镜。继续冲洗。
P362+P364 : 脱掉沾污的衣服,清洗后方可重新使用。
P332 + P313 : 如发生皮肤刺激:求医/就诊。

相关法规

RTECS#DG9650000
281281J&K5,5'-Dithiobis(2-nitrobenzoic acid)99%78元/1G;   312元/5G;   1123元/25G;   4268元/100G;   展开

基本信息

分子式C14H8N2O8S2
分子量396.36
熔点240-245
MDL编码MFCD00007140
Beilstein1896821
EINECS 编号200-714-4

安全信息

图形或危害标志
提示语Warning
危险说明H315
H319
H335
防范说明P261
P305+P351+P338
WGK3
RTECSDG9650000
TSCA
A14331Alfa Aesar5,5'-Dithiobis(2-nitrobenzoic acid)99%430元/1g;   1047元/5g;   4782元/25g;   展开

应用
Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.

备注
Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong oxidizing agents and strong bases.

基本信息

MDL
MFCD00007140

EINECS
200-714-4

分子式
C14H8N2O8S2

分子量
396.36

熔点
ca 240° dec.

灵敏度
Ambient temperatures.

形态
Crystalline solid

溶解性
Soluble in water and ethanol.

Beilstein
1896821

GHS危害和防范说明

GHS符号

Hazard Statements
H315-H319-H335
Causes skin irritation.Causes serious eye irritation.May cause respiratory irritation.

Precautionary Statements
P261-P280a-P305+P351+P338-P304+P340-P405-P501a
Avoid breathing dust/fume/gas/mist/vapours/spray.Wear protective gloves and eye/face protection.IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.Store locked up.Dispose of contents/container in accordance with local/regional/national/international regulations.

安全信息

危险类别
36/37/38
Irritating to eyes, respiratory system and skin.

安全等级
26-37-60
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.This material and its container must be disposed of as hazardous waste

RTECS
DG9650000

TSCA

C11754Alfa Aesar5,5'-Dithiobis-(2-nitrobenzoic acid)99%690元/1g;   956元/5g;   4726元/25g;   展开

基本信息

Ref#
11754

MDL
7140

分子式
C14 H8 N2 O8 S2

分子量
396.34

形态
Yellow crystalline solid

D8130Sigma-Aldrich5,5′-Dithiobis(2-nitrobenzoic acid)≥98%, BioReagent, suitable for determination of sulfhydryl groups270元/500 MG;   583.53元/1 G;   1423.24元/5 G;   2632.92元/10 G;   5354.75元/25 G;   21294.4元/100 G;   展开

产品说明

一般描述

5,5′双二硫代(2-硝基苯甲酸)也称为DTNB或Ellman′s试剂。这是一种与巯基相互作用的水溶性试剂,在中性pH下方便地应用。硫代阴离子(RS-)与Ellman′s试剂(DTNB2-)反应生成2-硝基-5-硫代苯甲酸盐阴离子(TNB2-)和一种混合二硫化物(R-S-TNB-)。TNB2- 具有较高的摩尔吸收系数,在412nm处有吸收。这种吸收与pH无关,在pH>7.3。

应用

Ellman′s试剂可用于乙酰胆碱酯酶的检测,并可对半胱氨酸残基进行特异性标记。该试剂是测定蛋白质、多肽和组织中游离巯基含量的敏感试剂。

包装

500 mg in glass insert
1, 5, 10, 25, 100 g in poly bottle

基本信息

线性分子式[-SC6H3(NO2)CO2H]2
分子量396.35
Beilstein1896821
EC 号200-714-4
MDL编号MFCD00007140
PubChem化学物质编号24894189
NACRESNA.26

产品性质

质量水平200
产品线BioReagent
测定≥98%
形式powder
mp240-245 ℃ (dec.) (lit.)
适用性suitable for determination of sulfhydryl groups
环保替代产品分类Re-engineered
SMILES stringOC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O
InChI1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
InChI keyKIUMMUBSPKGMOY-UHFFFAOYSA-N

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
个人防护装备dust mask type N95 (US), Eyeshields, Gloves
D218200Sigma-Aldrich5,5′-Dithiobis(2-nitrobenzoic acid)ReagentPlus®, 99%438.65元/1 G;   1187.42元/5 G;   2357.64元/10 G;   展开

产品说明

应用

5,5′-二硫代双(2-硝基苯甲酸)是一种水溶性试剂,主要用于巯基的衍生化。

包装

1, 5 g in glass bottle
10 g in poly bottle

法律信息

ReagentPlus is a registered trademark of sigma-aldrich Co. LLC

基本信息

线性分子式[-SC6H3(NO2)CO2H]2
分子量396.35
Beilstein1896821
EC 号200-714-4
MDL编号MFCD00007140
PubChem化学物质编号24893651

产品性质

质量水平200
产品线ReagentPlus®
测定99%
mp240-245 ℃ (dec.) (lit.)
SMILES stringOC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O
InChI1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
InChI keyKIUMMUBSPKGMOY-UHFFFAOYSA-N

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
个人防护装备dust mask type N95 (US), Eyeshields, Gloves
322123Sigma-Aldrich5,5'-Dithio-bis-(2-nitrobenzoic Acid)-CAS 69-78-3-CalbiochemSulfhydryl reagent used to characterize reactive SH groups.1640.36元/5 G;   展开

产品说明

一般描述

Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.
Sulfhydryl reagent used to characterize reactive SH groups. Useful in photometric determination of thiols.

包装

5 g in Glass bottle
Packaged under inert gas

生化/生理作用

Product does not compete with ATP.
Reversible: no
Primary Target
Useful in photometric determination of thiols
Cell permeable: no

警告

Toxicity: Standard Handling (A)

制备说明

May require gentle heating for complete solubilization.

其他说明

Ellman, G.L., et al. 1961. Biochem. Pharmacol.7, 88.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C14H8N2O8S2
分子量396.35
MDL编号MFCD00007140

产品性质

质量水平100
测定≥98% (titration)
形式solid
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
desiccated
protect from light
颜色 yellow
溶解性ethanol: 8 mg/mL
运输ambient
储存温度2-8℃
InChI1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
InChI keyKIUMMUBSPKGMOY-UHFFFAOYSA-N

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
V900454Sigma-Aldrich5,5′-Dithiobis(2-nitrobenzoic acid)Vetec, reagent grade, 98%405.64元/5 G;   展开

产品说明

包装

5, 25 g in poly bottle

法律信息

Vetec is a trademark of sigma-aldrich International GmbH

基本信息

线性分子式[-SC6H3(NO2)CO2H]2
分子量396.35
Beilstein1896821
EC 号200-714-4
MDL编号MFCD00007140
PubChem化学物质编号329829856

产品性质

等级reagent grade
产品线Vetec
测定98%
mp240-245 ℃ (dec.) (lit.)
SMILES stringOC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O
InChI1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
InChI keyKIUMMUBSPKGMOY-UHFFFAOYSA-N

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
967209Amethyst5,5'-Dithiobis(2-nitrobenzoic acid)99%, 试剂级56元/1G;   223元/5G;   891元/25G;   2934元/100G;   展开

基本信息

分子式C14H8N2O8S2
分子量396.36
熔点240-245
MDL编码MFCD00007140
Beilstein1896821
EINECS 编号200-714-4

安全信息

图形或危害标志
提示语Warning
危险说明H315
H319
H335
防范说明P261
P305+P351+P338
WGK3
RTECSDG9650000
TSCA
047885FluorochemDTNB98%264元/1g;   572元/5g;   990元/10g;   2266元/25g;   展开

基本信息

CAS Number69-78-3
MDL NumberMFCD00007140
Melting Point243-245℃

安全信息

图形或危害标志
危险性说明 H315: Causes skin irritation.
H319: Causes serious eye irritation.
H335: May cause respiratory irritation.
防范说明 P233: Keep container tightly closed.
P260: Do not breathe .
P261: Avoid breathing .
P264: Wash hands thoroughly after handling.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear .
P302 + P352: IF ON SKIN: Wash with plenty of soap and water.
P304: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing..
P304 + P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P312: Call a POISON CENTER or doctor/physician if you feel unwell.
P321: Specific treatment (see relevant procedures. on this label).
P332 + P313: If skin irritation occurs: Get medical advice/ attention.
P337 + P313: If eye irritation persists: Get medical advice/attention.
P340: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P362: Take off contaminated clothing and wash before reuse.
P403: Store in a well-ventilated place.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
P405: Store locked up.
P501: Dispose of contents/container to in accordance with local regulation .
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