产品说明

一般描述

单磷酸腺苷蛋白激酶(AMPK)激活剂5-氨基咪唑-4-羧酰胺核糖核苷酸(AICAR)可调节细胞能量。它可调控脂质和葡萄糖代谢、促炎反应、细胞因子生产、细胞增殖和凋亡。AICAR可改善大鼠的缺血或再灌注损伤和肾脏纤维化。其可提供肾小管坏死保护作用。

应用

AICAR已用于通过JAK(酪氨酸蛋白激酶)/ STAT(信号转导和转录激活子)/ SOCS(细胞因子信号转导抑制剂)途径提供抗顺铂诱导的急性肾脏损伤保护。它还用于检测FapR(丙二酰辅酶A反应性细菌转录因子)-NLuc(纳米荧光素酶)生物传感器的活性。

包装

5, 25 mg in glass bottle

生化/生理作用

阿卡地新是AMP活化蛋白激酶(AMPK)的细胞渗透性激活剂，是一种代谢主调节因子，在能量利用率降低时被激活(高细胞AMP:ATP比值)，用于抑制合成代谢过程。在体内，药物激活AMPK与AICAR可模拟运动和触发胰岛素无关的骨骼肌葡萄糖摄取。

特点和优势

该化合物是激酶磷酸酶生物学研究的推荐产品。点击此处查看更多特色的激酶磷酸酶生物学产品。在sigma.com/discover-bsm 上了解有关生物活性小分子在其他研究领域的更多信息。

基本信息

产品性质

安全信息

产品说明

一般描述

A cell-permeable nucleoside compound whose phosphorylated metabolite activates adenosine monophosphate-activated protein kinase (AMPK) and acts as a regulator of de novo purine synthesis. Stimulates glucose uptake in perfused and isolated muscle. Offers protection against cell death induced by glucose deprivation, chemical hypoxia, and exposure to glutamate and Aβ peptide.
A cell-permeable nucleoside compound that is processed intracellularly to form a phosphorylated metabolite, which activates adenosine monophosphate-activated protein kinase (AMPK) without disrupting the cellular concentrations of ATP, ADP, or AMP. Also acts as a regulator of de novo purine synthesis. Stimulates glucose uptake in both perfused and isolated muscle. AICAr-stimulated glucose transport is not affected by Wortmannin (Cat. No. 681675), a PI-3K inhibitor. Shown to inhibit the synthesis of triacylglycerol (TAG), diacylglycerol (DAG), and phospholipid, probably as a result of AMPK activation and the subsequent inhibition of sn-glycerol-3-phosphate acyltransferase (GPAT) by AMPK. Also reported to inhibit Hsp90 chaperone function. Imparts protection against cell death induced by glucose deprivation, chemical hypoxia, and exposure to glutamate and amyloid β (Aβ) peptide.

包装

50 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Adenosine monophosphate-activated protein kinase (AMPK)

警告

Toxicity: Standard Handling (A)

制备说明

May require heating to 50°C to achieve complete solubilization in methanol.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Meli, M., et al. 2006. J. Med. Chem.in press.
Culmsee, C., et al. 2001. J. Mol. Neurosci.17, 45.
Muoio, D.M., et al. 1999. Biochem. J.338, 783.
Hayashi, T., et al. 1998. Diabetes47, 1369.
Corton, J.M., et al. 1995. Eur. J. Biochem.229, 558.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

产品性质

安全信息